川芎天麻对偏头痛治疗作用的动物实验研究  

Animal Experimental Study on the Preventive Effect of Chuanxiong-Tianma on Migraine

作　　者：水俊涛 韦钦 章正祥[1] 张丽萍[1] 黄春华[3] 侯群[1] Shui Juntao

机构地区：[1]浙江中医药大学附属第一医院,310006 [2]浙江省东阳市人民医院,322103 [3]江西中医药大学附属医院,330006

出　　处：《浙江临床医学》2024年第8期1116-1118,1122,共4页Zhejiang Clinical Medical Journal

基　　金：浙江省自然科学基金项目(LY18H270009);浙江中医药大学校级科研基金项目(2020ZG40)。

摘　　要：目的探讨川芎天麻对大鼠偏头痛的治疗作用及对硬脑膜肥大细胞脱颗粒的影响。方法将28只雄性SD大鼠随机分为对照组、模型组、川芎天麻组和托吡酯组,每组7只。模型组与对照组超纯水灌胃,川芎天麻组川芎天麻颗粒(4∶1)灌胃,托吡酯组托吡酯溶液灌胃,持续1周。对照组腹腔注射0.9%氯化钠溶液,其余组腹腔注射硝酸甘油建立偏头痛大鼠模型。记录各组60~90 min的挠头次数和是否存在耳红症状,比较各组肥大细胞脱颗粒阳性率。结果川芎天麻颗粒能改善硝酸甘油诱导偏头痛大鼠的挠头症状(P<0.05),同时还能抑制肥大细胞脱颗粒(P<0.05)。结论川芎天麻和托吡酯均对硝酸甘油诱导的偏头痛大鼠头痛起到一定的治疗作用,可能与其抑制肥大细胞脱颗粒有关。Objective To explore the preventive effect of Chuanxiong-Tianma on headache and the degranulation of dural mast cells(MC)in migraine rats induced by nitroglycerin.Methods 28 healthy male SD rats stochastically put into the control group,the model group,the Chuanxiong Tianma group and the topiramate group,with 7 rats in each group.The rats in the control group and model group were given ultra-pure water for one week.The rats in the Chuanxiong Tianma group were treated with Chuanxiong-Tianma(4∶1)granules for 1 week,and the topiramate group were treated with topiramate solution for 1 week.One week after intragastric administration,apart from the control group,the other three groups were intraperitoneal injection with nitroglycerin to build the migraine model.The number of head scratches and the presence of ear red symptoms were calculated between 60 and 90 minutes in each group.The dural mast cells were treated with toluidine blue staining,and the positive rate of dural mast cells degranulation was compared between each group.Results Chuanxiong Tianma granules could improve the symptoms of head scratching in rats with migraine induced by nitroglycerin(P<0.05),and inhibit mast cell degranulation(P<0.05).Conclusion Chuanxiong Tianma and topiramate have certain preventive effect on migraine headache induced by nitroglycerin in rats,which may be related to their inhibition of mast cell degranulation.

关 键 词：偏头痛 肥大细胞 脱颗粒 硬脑膜 

分 类 号：R285.5[医药卫生—中药学]