Detection and Identification of Phytoplasma of Cleome rutidosperma in Areca Palm Yellow Leaf Disease Field  

作　　者：Zhaowei LIN Xiaoqing NIU Shida LONG Qinghua TANG Dejie YANG Weiwei SONG 

机构地区：[1]Coconut Research Institute of Chinese Academy of Tropical Agricultural Sciences/Hainan Innovation Center of Academician Team,Wenchang 571339,China

出　　处：《Plant Diseases and Pests》2024年第4期7-12,共6页植物病虫害研究（英文版）

基　　金：Supported by Innovation Platform for Academicians of Hainan Province of China(YSPTZX202151,YSPTZX202138);Hainan Provincial Natural Science Foundation of China(321QN345).

摘　　要：[Objectives]The paper was to detect and identify the phytoplasma of Cleome rutidosperma in areca palm yellow leaf disease(YLD)field in Wenchang City,Hainan Province,China.[Methods]The nested PCR technique was employed to amplify the phytoplasma 16S rDNA of C.rutidosperma samples,followed by sequence analysis.Concurrently,this study examined C.rutidosperma in YLD field,collecting symptomatic leaves for phytoplasma detection.[Results]The 16S rDNA sequence of the C.rutidosperma witches'-broom phytoplasma was found to be identical to that of the HNWC5 strain associated with areca palm yellows phytoplasma,leading to the identification of this phytoplasma as belonging to the 16SrII-A subgroup.Field investigations revealed a higher incidence of C.rutidosperma in areca palm fields,with symptoms of leaf yellows observed in six of these fields.Quantitative PCR(qPCR)analysis confirmed the presence of phytoplasma infection in these instances.[Conclusions]Through the analysis of geographical distribution,sequence alignment,and field occurrence data,a significant correlation has been identified between witches'broom disease and YLD.It is proposed that the former may act as an intermediate host for the areca palm yellows phytoplasma.

关 键 词：Areca palm yellow leaf disease PHYTOPLASMA Cleome rutidosperma IDENTIFICATION Detection 

分 类 号：S763.7[农业科学—森林保护学]