Fe_(3)O_(4)对降温冲击后厌氧氨氧化耦合羟基磷灰石系统的影响  

Effect of Fe_(3)O_(4) on the Anammox-hydroxyapatite system after temperature reduction

作　　者：王少坡 莫少庆[1,2,3] 刘灵婕 于静洁 毕艳孟 WANG Shaopo;MO Shaoqing;LIU Lingjie;YU Jingjie;BI Yanmeng(School of Environmental and Municipal Engineering,Tianjin Chengjian University,Tianjin 300384;Tianjin Key Laboratory of Aquatic Science and Technology,Tianjin 300384;Guangxi Tianyi Environmental Technology Co.,LTD,Qinzhou 535008)

机构地区：[1]天津城建大学环境与市政工程学院,天津300384 [2]天津市水质科学与技术重点实验室,天津300384 [3]广西天宜环境科技有限公司,钦州535008

出　　处：《环境科学学报》2024年第9期182-193,共12页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.42307178);天津市自然科学基金项目(No.18JCZDJC10080);天津市研究生科研创新项目(No.2022SKYZ172)。

摘　　要：在升流式厌氧污泥床(Upflow anaerobic sludge bed,UASB)反应器中,研究了外加Fe_(3)O_(4)对厌氧氨氧化-羟基磷灰石(Anaerobic ammonium oxidation-hydroxyapatite,Anammox-HAP)系统受降温冲击后的影响和作用机制.结果表明,当温度从30℃降低到17℃后,氮去除效率(Nitrogen removal efficiency,NRE)从90.25%下降到46.25%,当以10 d为更换周期投加200 mg⸱L-1的Fe_(3)O_(4)时,NRE提升到55.54%.分析发现,投加Fe_(3)O_(4)提高了系统中heme c含量(增加24.75%)和电子传递系统活性(提升46.88%);在氮代谢酶活性方面,200 mg⸱L-1 Fe_(3)O_(4)的投加使得亚硝酸盐还原酶(Nitrite reductase,NIR)和联氨合成酶(Hydrazine synthase,HZS)活性分别提高了75.00%和190.91%,但联氨氧化酶(Hydrazine oxidase,HZO)活性下降了47.62%.微生物群落分析显示,Candidatus Brocadia为优势厌氧氨氧化细菌(Anammox bacteria,AnAOB),其相对丰度大于20.40%,且与Fe_(3)O_(4)投加量呈正相关.此外,Fe_(3)O_(4)投加后,氮代谢基因丰度增加,其中,nxrB、hzo和hzsB基因的丰度分别增加了81.81%、31.99%和41.70%.宏基因组分析发现,在降温到17℃后,投加200 mg⸱L-1的Fe_(3)O_(4)使得AnAOB中参与Fe(III)代谢、细胞色素c氧化还原酶和生物醌池合成的基因相对丰度升高.总之,在受降温冲击后,外加Fe_(3)O_(4)不仅提高了Anammox-HAP系统内关键氮代谢酶(NIR和HZS)的活性,还增强了AnAOB的电子传递能力和Fe(III)利用能力,从而提升了系统受降温冲击后的脱氮性能.An upflow anaerobic sludge bed(UASB)reactor was employed to study the effects and mechanisms of Fe_(3)O_(4) addition on the anaerobic ammonium oxidation-hydroxyapatite(Anammox-HAP)system after a temperature reduction shock.When the temperature decreased from 30℃to 17℃,the nitrogen removal efficiency(NRE)declined from 90.25%to 46.25%.After subsequent additions of 200 mg·L-1 Fe_(3)O_(4) every ten days,the NRE increased to 55.54%.The analysis results indicated that Fe_(3)O_(4) supplementation increased the heme c content(by 24.75%)and the electron transfer system activity(by 46.88%)of the system.Regarding nitrogen metabolism enzyme activity,the addition of 200 mg·L-1 Fe_(3)O_(4) increased the activities of nitrite reductase(NIR)and hydrazine synthase(HZS)by 75.00%and 190.91%,respectively,whereas it reduced the activity of hydrazine oxidoreductase(HZO)by 47.62%.Microbial community analysis identified that Candidatus Brocadia was the dominant anaerobic ammonium-oxidizing bacteria(AnAOB)with a relative abundance exceeding 20.40%,and it was positively correlated with Fe_(3)O_(4) dosage.Following the addition of Fe_(3)O_(4),an increase in the abundance of nitrogen metabolism genes was observed,with nxrB,hzo,and hzsB gene abundances rising by 81.81%,31.99%,and 41.70%,respectively.After the temperature was reduced to 17℃,metagenomic analysis revealed that the addition of 200 mg·L-1 Fe_(3)O_(4) led to an increased relative abundance of genes involved in Fe(III)metabolism,cytochrome c oxidoreductase synthesis,and bioquinone pool formation in AnAOB.In summary,after temperature reduction shock,Fe_(3)O_(4) supplementation not only enhanced the activity of key nitrogen metabolism enzymes(NIR and HZS)within the Anammox-HAP system but also strengthened the electron transfer capabilities and Fe(III)utilization of AnAOB,which further improved the nitrogen removal performance of the anammox-HAP system.

关 键 词：厌氧氨氧化 Fe_(3)O_(4) 降温冲击 宏基因组分析 

分 类 号：X703.1[环境科学与工程—环境工程]