初始pH对AQS介导剩余污泥暗发酵产氢特性的影响  

The effect of initial pH on AQS-mediated biohydrogen production from waste activated sludge by dark fermentation

作　　者：张立国[1] 宁艺璇 刘琦 李建政[2] 白雅楠 岳嘉欣 班巧英[1] ZHANG Liguo;NING Yixuan;LIU Qi;LI Jianzheng;BAI Yanan;YUE Jiaxin;BAN Qiaoying(College of Environment and Resource,Shanxi University,Taiyuan 030006;State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090)

机构地区：[1]山西大学环境与资源学院,太原030006 [2]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090

出　　处：《环境科学学报》2024年第9期311-318,共8页Acta Scientiae Circumstantiae

基　　金：山西省基础研究计划资助项目(No.202203021211314);哈尔滨工业大学城市水资源与水环境国家重点实验室开放基金(No.QA202137)。

摘　　要：为探讨pH对蒽醌-2-磺酸钠(AQS)介导剩余污泥暗发酵产氢的影响,本研究采用间歇方式考察了中温条件(35℃)下,初始pH(4.5~7.0)对AQS介导剩余污泥暗发酵产氢效能的影响,并通过Illumina MiSeq测序揭示了暗发酵产氢相关微生物的演替规律.结果表明,在初始pH 4.5~7.0范围内,AQS介导剩余污泥暗发酵产氢效能随pH提高呈先增加后降低再增加的变化趋势.其中,在初始pH 6.0时,剩余污泥的产氢效能最佳,其累计产氢量和最大产氢速率(Rmax)分别为41.9 mL·g^(-1)和16.2 mL·g^(-1)·d^(-1).在该条件下,挥发酸总量也达到最高值590.9 mg·L^(-1),其中乙酸占63.8%.Illumina MiSeq测序揭示了暗发酵系统中优势菌群的丰度存在一定差异,Longilinea、Hyphomicrobium和Pelospora在初始pH 6.0条件下富集,相对丰度分别达到了21.7%、2.6%和1.5%.冗余分析(RDA)表明,氢气产量与Longilinea、Aminivibrio、Guggenheimella、Proteiniclasticum、Pirellula、Youngiibacter、Gimesia和Hyphomicrobium呈正相关性.To reveal the effect of pH on biohydrogen production from waste activated sludge(WAS)by anthraquinone-2-sulfonate(AQS)mediating,the biohydrogen production of WAS at varied initial pH(4.5~7.0)within mesophilic condition(35℃)were investigated by batch test.The shift of the microorganisms related to biohydrogen production by dark fermentation was revealed based on Illumina MiSeq sequencing.Results showed that increasing the initial pH from 4.5 to 7.0,the biohydrogen production of WAS by AQS mediating exhibited a three-phase kinetics:an initial increase,followed by a decrease and a further increase.At the initial pH of 6.0,the yield of biohydrogen reached optimal performance,where the accumulative biohydrogen production and maximum biohydrogen production rate(Rmax)were 41.9 mL·g^(-1)and 16.2 mL·g^(-1)·d^(-1),respectively.Under such conditions,the yield of volatile fatty acids also reached the maximum(590.9 mg·L^(-1)),in which the acetate accounted for 63.8%of the overall volatile fatty acids.The results of Illumina MiSeq sequencing showed that the relative abundance of dominant microbial groups in fermentation systems was different.At the initial pH of 6.0,Longilinea,Hyphomicrobium and Pelospora enriched and their relative abundance reached 21.7%,2.6%and 1.5%,respectively.Redundancy analysis(RDA)indicated that Longilinea,Aminivibrio,Guggenheimella,Proteiniclasticum,Pirellula,Youngiibacter,Gimesia and Hyphomicrobium played a positive role on biohydrogen production.

关 键 词：剩余污泥 初始pH 蒽醌-2-磺酸钠 产氢量 微生物群落 

分 类 号：X703.5[环境科学与工程—环境工程]