遵义市59株人源沙门菌分型及耐药研究  

Typing and antimicrobial resistance testing of Salmonella isolates from diarrhea patients in Zunyi

作　　者：韦德琴 曹家艳[1] 粟绍翾 郑君 董昰 王铭 赵明伍 WEI Deqin;CAO Jiayan;SU Shaoxuan;ZHENG Jun;DONG Xia;WANG Ming;ZHAO Mingwu(Department of Laboratory,Zunyi Center for Disease Control and Prevention,Zunyi,Guizhou 563000,China;Department of Laboratory,Guizhou Center for Disease Control and Prevention,Guiyang,Guizhou 550000,China)

机构地区：[1]遵义市疾病预防控制中心检验科,贵州遵义563000 [2]贵州省疾病预防控制中心检验科,贵州贵阳550000

出　　处：《热带医学杂志》2024年第8期1100-1104,1158,F0004,共7页Journal of Tropical Medicine

基　　金：贵州省科技厅计划项目(黔科合基础-ZK[2024]一般681);中央高校基本科研业务费专项资金项目(3332022158);贵州省卫生健康委资金项目(gzwkj2021-434);遵义市科技创新团队项目[2022]9号。

摘　　要：目的了解遵义市腹泻患者沙门菌分型及耐药特征,为防控提供理论依据。方法收集2020-2022年遵义市腹泻患者沙门菌菌株,运用玻片凝集法进行血清分型,脉冲场凝胶电泳(PFGE)进行分子分型,利用肉汤稀释法进行耐药监测,应用BioNumerics软件进行数据分析。结果59株人源沙门菌鉴定为8种血清型,以鼠伤寒沙门菌和肠炎沙门菌为主,分别占52.54%和33.90%;PFGE带型呈现多态性,存在6族13株100%同源菌株和8族19株高度同源菌株;药敏结果显示对17种抗生素存在耐药的菌株占93.22%,多重耐药率达76.27%。其中对氨苄西林、链霉素、四环素的耐药性较高,对阿米卡星、替加环素、厄他培南、美罗培南、头孢他啶/阿维巴坦、阿奇霉素较为敏感;鼠伤寒沙门菌对链霉素、氨苄西林和四环素高耐药,肠炎沙门菌对氨苄西林和萘啶酸高耐药。结论遵义市人源沙门菌优势血清型为鼠伤寒沙门菌和肠炎沙门菌,具有遗传多样性,对抗生素的耐药率高,多重耐药严重,不同血清型耐药率存在差异,需加强对多重耐药株的监控及抗生素使用的监管。Objective Investigate the typing and drug resistance characteristics of Salmonella isolates in diarrhea patients in Zunyi city.Methods Serotyping of Salmonella strains isolated from diarrhea patients in Zunyi from 2020 to 2022 was performed by serum agglutination method,and molecular typing was performed by pulsed⁃field gel electrophoresis(PFGE);drug resistance was determined by broth dilution method.BioNumerics software was used for result analysis.Results 59 strains of human Salmonella were identified into 8 serotypes,with Salmonella typhimurium and Salmonella enteritidis accounting for 52.54%and 33.90%,respectively;PFGE banding showed polymorphism,with 13100%homologous strains in 6 families and 19 highly homologous strains in 8 families;drug sensitivity results showed that 93.22%of the strains were resistant to 17 antibiotics,and the multidrug resistance rate reached 76.27%.Among them,the resistance to ampicillin,streptomycin and tetracycline was high,and it was relatively sensitive to amikacin,tigecycline,ertapenem,meropenem,ceftazidime/avibactam and azithromycin;Salmonella typhimurium was highly resistant to streptomycin,ampicillin and tetracycline,and Salmonella enteritidis was highly resistant to ampicillin and nalidixic acid.Conclusions The dominant serotypes of human Salmonella in Zunyi city were Salmonella typhimurium and Salmonella enteritidis,which were genetically diverse,had a high resistance rate to antibiotics,and had serious multidrug resistance.There were differences in the resistance rates of different serotypes,and the monitoring of multidrug⁃resistant strains and the supervision of the use of antimicrobial drugs should be strengthened.

关 键 词：腹泻 沙门菌 血清分型 脉冲场凝胶电泳 耐药性 

分 类 号：R446.5[医药卫生—诊断学]