霍山石斛对腹泻型肠易激综合征小鼠肠道菌群多样性的影响  

Effect of Dendrobium huoshanense on the diversity of gut microbiota in mice with diarrhea induced irritable bowel syndrome

作　　者：陈涛 林彩霞 王成 马猛华 兰俞 韩邦兴[2,3] 刘东 苏婷[2,3] CHEN Tao;LIN Caixia;WANG Cheng;MA Menghua;LAN Yu;HAN Bangxing;LIU Dong;SU Ting(School of Pharmacy,Anhui University of Chinese Medicine,Hefei,Anhui 230012,China;College of Biological and Pharmaceutical Engineering,West Anhui University,Lu'an,Anhui 237012,China;不详)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]皖西学院生物与制药工程学院 [3]安徽省中药生态农业工程研究中心 [4]霍山县天下泽雨生物科技发展有限公司

出　　处：《中国微生态学杂志》2024年第8期869-875,共7页Chinese Journal of Microecology

基　　金：国家“十四五”重点研发计划课题(2023YFC3503804);安徽省大别山中医药研究院开放课题重点科技攻关项目(TCMADM-2023-02,TCMADM-2023-08);安徽省中药生态农业工程研究中心开放课题(WXZR202316);皖西学院高层次人才基金项目(WGKQ2021083,WGKQ2022074);国家级、安徽省大学生创新创业训练项目(202310376037,s202310376123);安徽省中药资源保护与持续利用工程实验室开放课题(TCMRPSU-2022-01)。

摘　　要：目的探讨霍山石斛对腹泻型肠易激综合征(diarrhea predominant-irritable bowel syndrome,IBS-D)小鼠肠道菌群的影响,为霍山石斛对IBS-D的疗效机制研究提供依据。方法将24只SPF级雄性小鼠随机分为正常组、模型组、霍山石斛组、匹维溴铵组,每组6只。采用番泻叶灌胃结合束缚夹尾应激的方法制备IBS-D模型。造模成功后,霍山石斛组和匹维溴铵组分别给予相应药物剂量灌胃,持续1周。比较各组小鼠一般情况、粪便性状、粪便含水率、腹部撤退反射评分以及采用16S rRNA基因高通量测序检测小鼠肠道菌群种类和丰度的变化。结果与正常组相比,IBS-D模型小鼠精神欠佳,排便量增多,粪便含水率和内脏敏感度均升高,肠道菌群多样性和丰度有所下降;经霍山石斛水提物干预后,与模型组相比,霍山石斛组小鼠的精神状态有所改善,粪便性状恢复正常、粪便含水率和内脏敏感度均降低。物种相对丰度和组成结果表明霍山石斛水提物增加了IBS-D小鼠肠道菌群的多样性,并且显著增加了norank_Muribaculaceae、乳杆菌属、拟普雷沃菌属等肠道有益菌属的相对丰度,降低了变形菌门中埃希菌、志贺菌等肠道致病菌的相对丰度。结论霍山石斛降低了IBS-D小鼠的内脏高敏感,可能通过恢复IBS-D小鼠肠道菌群的多样性,调节肠道菌群相对丰度,发挥对IBS-D的治疗作用。Objective To observe the effects of Dendrobium huoshanense on the gut microbiota of mice with diarrhea predominant-irritable bowel syndrome(IBS-D),and provide a basis for the study of the therapeutic mechanism of Dendrobium huoshanense on IBS-D.Methods Twenty-four SPF grade male mice were randomly divided into normal control group,model group,Dendrobium huoshanense group,and pinaverium bromide group,with 6 mice in each group.The IBS-D model was prepared by gavage of senna leaves combined with restrained tail clamping stress.After successful modeling,the Dendrobium huoshanense group and pinaverium bromide group were given corresponding drug doses by gavage for one week.The general status,fecal characteristics,fecal moisture content,abdominal withdrawal reflex score were compared among groups;changes in gut microbiota species and abundance of mice in each group were detected using 16S rRNA gene high-throughput sequencing.Results Compared with the normal controls,the IBS-D model mice showed poor mental health,increased bowel movements,increased fecal moisture content and visceral sensitivity,and decreased diversity and abundance of gut microbiota.After intervention with Dendrobium huoshanense,compared with the model controls,the mental status of mice in the Dendrobium huoshanense group was improved,and the fecal characteristics returned to normal,while the fecal moisture content and visceral sensitivity decreased.The relative abundance and composition of species indicated that Dendrobium huoshanense increased the diversity of gut microbiota in IBS-D mice and significantly increased the relative abundances of beneficial bacteria genera such as norank_Muribaculaceae,Lactobacillus,Alloprevotella,and decreased those of pathogenic bacteria such as Escherichia and Shigella in the Proteobacteria phylum.Conclusion Dendrobium huoshanense reduce visceral hypersensitivity in IBS-D mice,possibly by restoring the diversity of gut microbiota in IBS-D mice,regulating the relative abundance of gut microbiota,and exerting therapeuti

关 键 词：霍山石斛 腹泻型肠易激综合征 肠道菌群 

分 类 号：R289[医药卫生—方剂学]