2型糖尿病个体粪菌液对不同饮食小鼠肠道菌群的影响及其与糖脂代谢的相关性  

Effects of fecal microbiota fluid from individuals with type 2 diabetes on intestinal flora of different diets mice and its correlation with glycolipid metabolism and inflammation

作　　者：王蟾月 王烨[3] 杨浩 谷娇娇 朱曼丽 李琳琳[3] WANG Chanyue;WANG Ye;YANG Hao;GU Jiaojiao;ZHU Manli;LI Linlin(School of Medicine,AnKang University,Ankang,Shaanxi 725000,China;不详)

机构地区：[1]安康学院医学院,陕西安康725000 [2]秦巴中药资源开发与利用陕西省高校工程研究中心 [3]新疆医科大学药学院 [4]新疆医科大学中心实验室

出　　处：《中国微生态学杂志》2024年第8期886-891,共6页Chinese Journal of Microecology

基　　金：国家自然科学基金地区项目(82360728);陕西省重点产业创新链项目(2019ZDLSF04-02);安康市科学技术研究发展计划项目(AK2023-SF-04)。

摘　　要：目的研究2型糖尿病(T2DM)粪菌液对正常饮食和高脂饮食小鼠肠道菌群的影响,并分析肠道菌群与血糖血脂的相关性。方法40只雄性C57BL/6小鼠随机分为正常饮食组(ND组)、菌群移植组(MT组)、高脂饮食组(HFD组)与HFD+MT组,每组10只,T2DM人粪菌液干预8周。采用RT-qPCR测定粪便中多形拟杆菌、狄氏副拟杆菌、嗜黏蛋白阿克曼菌、普拉梭菌、嗜酸乳杆菌、大肠埃希菌数量,并分析菌群与餐后血糖(PPG)、空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、体重和炎症指标的相关性。结果四组小鼠粪便样本中大肠埃希菌、普拉梭菌、嗜酸乳杆菌相对丰度存在显著差异(F=8.081,P<001;F=17.353,P<001;F=7.181,P<001)。与ND组比,MT、HFD、HFD+MT组大肠埃希菌水平升高,普拉梭菌及嗜酸乳杆菌水平降低。与HFD组比较,HFD+MT组大肠埃希菌升高,普拉梭菌降低。普拉梭菌与PPG、FBG、TC、脂多糖结合蛋白(LBP)显著相关(r=-0.585,r=-0.639,r=-0.716,r=-0.626,均P<0.05),大肠埃希菌与PPG、FBG、TC、肿瘤坏死因子α(TNF-α)、LBP显著相关(r=0.455,r=0.545,r=0.456,r=0.451,r=0.433,均P<0.05),嗜酸乳杆菌与FBG、TC、TNF-α、LBP显著负相关(r=-0.583,r=-0.497,r=-0.565,r=-0.614,均P<0.05)。结论T2DM人粪菌移植可能通过改变正常饮食或高脂饮食小鼠大肠埃希菌、普拉梭菌及嗜酸乳杆菌水平,使肠道菌群失衡,引起炎症,进而导致糖脂代谢紊乱。Objective To explore the effect of type 2 diabetes(T2DM)fecal bacteria on intestinal flora of normal and high-fat diet mice,and analyze the correlation of intestinal flora with blood glucose,lipid and inflammation.Methods Forty male C57BL/6 mice were randomly divided into four groups:the normal diet(ND)group,the microbiota transplantation(MT)group,the high-fat diet(HFD)group,and the HFD+MT group,with 10 mice in each group.These mice underwent an 8-week intervention with human fecal microbiota from T2DM patients.The copies of Bacteroides thetaiotaomicron,Parabacteroides distasonis,Akkermansia muciniphila,Faecalibacterium prausnitzii,Lactobacillus acidophilus and Escherichia coli in fecal samples were measured by RT-qPCR.The correlations between these species and post-prandial glucose(PPG),fasting blood glucose(FBG),total cholesterol(TC),triglycerides(TG)and inflammatory indices were analyzed.Results Significant differences in the relative abundances of E.coli,F.prausnitzii and L.acidophilus were observed among the four groups(F=8.081,P<001;F=17.353,P<001;F=7.181,P<001).Compared with the ND group,the MT group,HFD group and HFD+MT group showed increased level of E.coli and decreased levels of F.prausnitzii and L.acidophilus.Comparing with the HFD group and the HFD+MT group,the latter exhibited higher level of E.coli and lower level of F.prausnitzii.Notably,F.prausnitzii was significantly correlated with PPG,FBG,TC and LBP(r=−0.585,r=−0.639,r=−0.716,r=−0.626;all P<0.05).E.coli demonstrated significant correlations with PPG,FBG,TC,TNF-αand LBP(r=0.455,r=0.545,r=0.456,r=0.451,r=0.433;all P<0.05).Conversely,L.acidophilus showed significant negative correlations with FBG,TC,TNF-αand LBP(r=−0.583,r=−0.497,r=−0.565,r=−0.614;all P<0.05).Conclusion Fecal microbiota from patients with T2DM may disrupt the balance of intestinal microbiota in mice with normal or high-fat diets by altering the levels of E.coli,F.prausnitzii and L.acidophilus,inducing inflammation and subsequently causing glucose and lipid metab

关 键 词：菌群移植 2型糖尿病 肠道菌群 炎症 

分 类 号：R333.3[医药卫生—人体生理学]