一株巨菌草内生细菌分离鉴定及促生特性研究  

Isolation and Identification of an Endophytic Bacterium from a strain of JUJUNCAO and its Growth-promoting Properties

作　　者：刘天金 谢玮轩 易听雨 陈诗瑜 吴佳霓 杜蒙蒙 张春源 赖泽成 叶文雨[1,2,4] LIU Tianjin;XIE Weixuan;YI Tingyu;CHEN Shiyu;WU Jiani;DU Mengmeng;ZHANG Chunyuan;LAI Zecheng;YE Wenyu(China National Engineering Research Center of JUNCAO Technology,College of JunCao Science and Ecology(College of Carbon Neutrality),Fuzhou Fujian 350002,China;Fujian University Key Laboratory for Plant-Microbe Interaction,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou Fujian 350002,China;College of Future Technology(Haixia Institute of Science and Technology),Fujian Agriculture and Forestry University,Fuzhou Fujian 350002,China;Technology Innovation Center for Monitoring and Restoration Engineering of Ecological Fragile Zone in Southeast China,Ministry of NaturalResources,Fuzhou Fujian 350000,China)

机构地区：[1]福建农林大学国家菌草工程技术研究中心,菌草与生态学院(碳中和学院),福建福州350002 [2]植物与微生物相互作用福建省高校重点实验室,福建农林大学生命科学学院,福建福州350002 [3]福建农林大学未来技术学院(海峡联合研究院),福建福州350002 [4]自然资源部东南生态脆弱区监测修复工程技术创新中心,福建福州350000

出　　处：《青海农林科技》2024年第3期62-68,共7页Science and Technology of Qinghai Agriculture and Forestry

基　　金：福建省引导性科技计划项目(2022N0010);福建省自然资源科技创新项目资金资助(KY-090000-04-2022-016);福建农林大学大学生创新创业训练计划项目(X202310389126);福建省科技重大专项(2021NZ029009)。

摘　　要：本研究从巨菌草植物茎中分离出1株耐盐碱内生菌,对其进行了形态学观察和16S rRNA分子鉴定,还进行了生理生化和促生特性试验。结果显示,鉴定PJJ-1为泛菌属(Pantoea sp.),红色杆状,为革兰氏阴性菌。该菌株具有解磷、解硅酸盐、固氮、产铁载体等促生性能,在玉米和番茄的盆栽试验中具有一定的促生作用。本研究可为巨菌草的合理种植及生态治理提供参考。In this study,we isolated and molecularly identified a strain of salinity-tolerant endophyte from the stem of JUJUNCAO plant.Morphological observation and 16S rRNA molecalar identification were conducted on it,and physiological biochemical and growth promoting experiments were also carried out.The results showed that PJJ-1 was identified as a red rod\|shaped bacterium belonging to the Pantoea sp.genus,which was a Gram negative bacterium.This strain possessed the growth-promoting properties such as phosphorus solubilisation,silicate solubilisation,nitrogen fixation,and siderophore production,and has been shown to have some growth-promoting effects in potting experiments of maize and tomato.This study can provide a theoretical basis for the rational cultivation and ecological management of JUJUNCAO.

关 键 词：巨菌草 内生细菌 耐盐碱 生物学性状 

分 类 号：Q949.9[生物学—植物学]