The MADS-box gene RhAGL6 plays a master role in regulating the receptacle malformation in rose at low temperature  

作　　者：Weikun Jing Shuai Zhang Qingcui Zhao Yang Liu Liangjun Zhao Junping Gao Nan Ma Xiaofeng Zhou Yonghong Li 

机构地区：[1]School of Food and Drug,Shenzhen Polytechnic,Shenzhen 518055,China [2]Department of Ornamental Horticulture,College of Horticulture,China Agricultural University,Beijing 100193,China [3]Postdoctoral Innovation Practice Base,Shenzhen Polytechnic,Shenzhen 518055,China [4]Flower Research Institute of Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205,China

出　　处：《Horticultural Plant Journal》2024年第5期1214-1229,共16页园艺学报（英文版）

基　　金：the National Natural Science Foundation of China(Grant Nos.31972438,31902054,32202530);the Postdoctoral Initiation Project of Shenzhen Polytechnic(Grant Nos.6021330012K0,6020330006K0,and 6022312017K);Natural Science Foundation of Guangdong Province(Grant No.2021A1515110368);Major Agricultural Science and Technology Projects in Yunnan Province(Grant No.202102AE090052).

摘　　要：Low temperature usually results in the developmental deformity of flower organs,immensely affecting the quality of rose flowers.However,it's largely unknown about the regulatory mechanisms activated by low temperature.Here,we used a low temperature-sensitive Rosa hybrida cv.‘Peach Avalanche’to screen a MADS-box gene RhAGL6 via conjoint analysis between RNA sequencing(RNA-seq)and whole-genome bisulfite sequencing(WGBS).Furthermore,we found that low temperature induced the hypermethylation and elevated histone 3 lys-27 trimethylation(H3K27me3)level on the RhAGL6 promoter,leading to decreased RhAGL6 expression.In addition,RhAGL6 silencing resulted in the formation of abnormal receptacles.We also found that the levels of gibberellins(GA3)and abscisic acid(ABA)in the receptacle under low temperature were lower and higher,respectively,than under normal temperature.Promoter activity analysis revealed that GA3 significantly activated RhAGL6 promoter activity,whereas ABA inhibited it.Thus,we propose that RhAGL6 regulates rose receptacle development by integrating epigenetic regulation and phytohormones signaling at low temperature.

关 键 词：Rosa hybrida Low temperature RhAGL6 Receptacle development DNA methylation H3K27me3 PHYTOHORMONE 

分 类 号：S685.12[农业科学—观赏园艺]