紫草醌通过调控JNK信号通路抑制卵巢癌细胞增殖并诱导细胞凋亡  

Effect of Shikonin on Proliferation and Apoptosis of Ovarian Cancer Cells

作　　者：焦培英 杜巧前 李娜 王娇 JIAO Peiying;DU Qiaoqian;LI Na;WANG Jiao(Department of Obstetrics,Hengshui People’s Hospital,Hengshui,Hebei,053000,China;Department of General Surgery,Hengshui People’s Hospital,Hengshui,Hebei,053000,China;Department of Radiotherapy,Hengshui People’s Hospital,Hengshui,Hebei,053000,China;Department of Reproductive Medicine,Hengshui People’s Hospital,Hengshui,Hebei,053000,China)

机构地区：[1]衡水市人民医院产科,河北省衡水市053000 [2]衡水市人民医院普外科,河北省衡水市053000 [3]衡水市人民医院放疗科,河北省衡水市053000 [4]衡水市人民医院生殖医学科,河北省衡水市053000

出　　处：《医学分子生物学杂志》2024年第5期475-480,共6页Journal of Medical Molecular Biology

基　　金：衡水市重点研发计划项目(No.2023014072Z)。

摘　　要：目的探索紫草醌对卵巢癌细胞增殖、凋亡以及c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)信号通路表达的影响。方法将体外培养人卵巢癌细胞(SKOV-3)细胞分为对照组及紫草醌2.5、5和10μmol/L组。检测各组细胞增殖和凋亡情况,蛋白质印迹检测增殖细胞核抗原(PCNA)、裂解的半胱氨酸天冬氨酸蛋白酶9(cleaved Caspase-9)、半胱氨酸天冬氨酸蛋白酶3(Caspase-3)蛋白表达和c-Jun氨基末端激酶1/2(JNK1/2)的磷酸化。结果紫草醌对SKOV-3细胞增殖的抑制作用呈药物浓度依赖性,半抑制浓度(50%inhibiting concentration,IC50)=10.59μmol/L;与对照组比较,紫草醌中剂量组和紫草醌高剂量组细胞存活率和克隆形成率明显下降(P<0.05),细胞凋亡率明显升高(P<0.05),细胞中PCNA、(p-JNK1/2)/(JNK1/2)蛋白表达明显下降(P<0.05),cleaved Caspase-9/Caspase-9、cleaved Caspase-3/Caspase-3蛋白表达明显升高(P<0.05)。结论紫草醌可以抑制SKOV-3细胞增殖,抑制JNK信号通路,诱导细胞凋亡。Objective To explore the effect of shikonin on the proliferation and apoptosis of ovarian cancer cells and the expression of c-Jun N-terminal kinase(JNK)signaling pathway.Methods Human ovarian cancer cell(SKOV-3)cells cultured in vitro were divided into 4 groups:control group(0μmol/L),low-dose Shikonin group(2.5μmol/L),middle-dose Shikonin group(5μmol/L)and high-dose Shikonin group(10μmol/L).Cell proliferation and apoptosis were detected in each group.The expression levels of proliferating cell nuclear antigen(PCNA),cleaved Caspase-9 and Caspase-3 proteins,and c-Jun N-terminal kinase 1/2(JNK1/2)phosphorylation were detected by Western blotting.Results The inhibitory effect of shikonin on the proliferation of SKOV-3 cells showed dependence of drug concentration,with the semi-inhibitory concentration(IC50)of 10.59μmol/L.The cells survival rate and colony formation rate were significantly decreased in the middle-dose Shikonin group and high-dose Shikonin group(P<0.05),the apoptosis rate was significantly increased(P<0.05),the expression levels of PCNA and(p-JNK1/2)/(JNK1/2)were significantly decreased(P<0.05),and the levels of cleaved Caspase-9/Caspase-9 and cleaved Caspase-3/Caspase-3 were significantly increased(P<0.05),if compared with those in the control group.Conclusion Shikonin can inhibit the proliferation of SKOV-3 cells,inhibit JNK signaling pathway,and induce apoptosis.

关 键 词：卵巢癌 紫草醌 C-JUN氨基末端激酶 

分 类 号：R392.11[医药卫生—免疫学]