IgG型自身抗体Fab段遮断红细胞自身抗原的探索研究  

Exploration of the blocking effect of Fab fragment of IgG autoantibody on erythrocyte autoantigens

作　　者：黄海涛 温学红[1] HUANG Haitao;WEN Xuehong(Department of Medical Laborary,The Second Affiliated Hospital of Tianjin University of TCM,Tianjin 300250,China)

机构地区：[1]天津中医药大学第二附属医院检验科,天津300250

出　　处：《中国输血杂志》2024年第9期1077-1080,1090,共5页Chinese Journal of Blood Transfusion

基　　金：天津市输血协会科研项目(2021KY05)。

摘　　要：目的应用自身抗体的Fab段结合红细胞上的自身抗原,以遮断完整自身抗体与自身抗原的结合。方法含自身抗体的患者血清,通过吸收放散试验获得成分单一的自身抗体,再应用木瓜酶37℃过夜水解自身抗体IgG分子获得Fab段,用Fab段特异性结合相对应的自身抗原,从而遮断完整自身抗体与自身抗原的结合。结果3例含自身抗体的患者血浆标本,经过吸收放散试验获得的组分单一的自身抗体,均经过木瓜酶水解处理生成Fab段,此Fab段与各自放散液中的完整自身抗体混合后,均能遮断完整自身抗体与红细胞自身抗原的结合。同时3例标本之间,1号标本与2号标本的有相互遮断效应,而2号标本和3号标本之间未见相互遮断效应。结论自身抗体Fab段能消除完整自身抗体与红细胞自身抗原的凝集,此方法可以应用到临床实践中,解决多种临床问题。Objective To use Fab segments of autoantibodies to bind to autoantigens on red blood cells in order to block the binding of intact autoantibodies to the autoantigens.Methods The pure autoantibodies were obtained from the serum of patients by absorption and elution test,then Fab segments were obtained by hydrolyzing IgG autoantibodies at 37℃ overnight with papain enzyme.The Fab segments were used to bind the corresponding autoantigens specifically,thus blocking the binding of intact autoantibodies to autoantigens.Results The pure autoantibodies obtained through absorption and elution test were all hydrolyzed by papain to generate Fab fragments.When mixed with intact autoantibodies in their respective elution solutions,these Fab fragments were able to block the binding of intact autoantibodies to red blood cell autoantigens.Among the three samples,there was a mutual blocking effect between sample 1 and sample 2,while no mutual blocking effect was observed between sample 2 and sample 3.Conclusion Fab segment of autoantibody can eliminate the agglutination of intact autoantibodies and erythrocyte autoantigens,and can be applied to clinical practice to solve clinical problems.

关 键 词：自身抗体 FAB片段 遮断效应 

分 类 号：R457.1[医药卫生—治疗学]