构建永生化细胞的研究进展  

Progress in the construction of immortalized cells

作　　者：杨玥 顾海慧 YANG Yue;GU Haihui(Department of Transfusion Medicine,the First Affiliated Hospital of Naval Medical University,Shanghai 200433,China)

机构地区：[1]中国人民解放军海军军医大学第一附属医院输血科,上海200433

出　　处：《中国输血杂志》2024年第9期1081-1090,共10页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金面上项目(81970165)。

摘　　要：人类细胞在正常增殖分裂过程中会到达衰老期(M1期)和致死期(M2期),细胞会停止分裂并凋亡,这种不可逆的生理过程是机体内在的一种抗肿瘤机制。然而细胞有限的增殖能力限制了细胞在基础实验、临床应用和生物工程等领域的研究。建立稳定、持续增殖且结构功能正常的永生化细胞系是当前细胞生物学研究的热点和难点。永生化细胞也是生产工程化血细胞的重要来源。本综述总结了永生化细胞的构建技术和可逆转性永生化细胞的研究进展。Objective In the process of proliferation and division,normal human cells reach the mortality stage M1 and mortality stage M2,which makes the cells stop division and apoptosis.This irreversible physiological process is also an inherent anti-tumor mechanism.The limited ability of cell proliferation limits its role in basic research,clinical application,bioengineering and other fields.The development of immortalized cell lines with stable,continuous proliferation and normal structure and function has become a hot and difficult point in the research of cell biology.Immortalized cells are important sources for the production of engineered blood cells.This review discusses the molecular research process of immortalization technology which is widely used at present and describes the technology of immortalized cell de-immortalization.

关 键 词：永生化细胞 可逆转永生化 细胞衰老 端粒酶 工程化血细胞 

分 类 号：Q255[生物学—细胞生物学]