东京银背藤化学成分及其抗炎活性研究  

Chemical constituents from Argyreia pierreana and their anti-inflammatory activities

作　　者：滕明雪 刘嘉艺 钟金明 黄承鸿 廖广凤 卢汝梅 TENG Mingxue;LIU Jiayi;ZHONG Jinming;HUANG Chenghong;LIAO Guangfeng;LU Rumei(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200

出　　处：《中草药》2024年第15期5018-5023,共6页Chinese Traditional and Herbal Drugs

基　　金：广西科技基地和人才专项(桂科AD20159007);广西中医药大学桂派中医药传承创新团队资助项目(2022B005);2022年自治区中药学研究生联合培养基地开放项目(桂学位[2021]6号);2022年大学生创新创业训练计划项目(S202210600051);2021年大学生创新创业训练计划项目(S202110600113)。

摘　　要：目的研究东京银背藤Argyreia pierreana的化学成分及其抗炎活性。方法运用硅胶、SephadexLH-20、C18等柱色谱和半制备高效液相色谱对其化学成分进行分离纯化,根据MS、1D/2D NMR等波谱技术确定化合物结构。采用Griess试剂法测试化合物抗炎活性。结果从东京银背藤中分离得到13个化合物,分别鉴定为8-羟基-1,9-二甲氧基-[2′,2′-二甲基吡喃(5′,6′:2,3)]香豆酮并色酮(1)、derrusin(2)、东莨菪内酯(3)、caesalpiniaphenol D(4)、(-)-gynuraone(5)、咖啡酸甲酯(6)、咖啡酸乙酯(7)、阿魏酸乙酯(8)、反式对羟基肉桂酸乙酯(9)、对羟基苯甲酸(10)、香草酸(11)、咖啡酸(12)、阿魏酸(13)。结论化合物1为新的苯并呋喃[3,2-c]香豆素类化合物,命名为银背藤内酯(argypierstan),化合物2、4~6、8~13为首次从东京银背藤分离得到。化合物4、6、8和9具有较好的抗炎活性,半数抑制浓度(half maximal inhibitory concentration,IC_(50))分别为(43.38±0.44)、(4.95±0.10)、(35.96±0.31)、(42.77±1.80)μmol/L。Objective To study the chemical components and their anti-inflammatory activities in Argyreia pierreana.Methods The chemical components were isolated and purified using methods including silica gel,Sephadex LH-20,C18 column chromatography and semi-preparative high performance liquid chromatography.The structures of the compounds were identified by MS,1D/2D NMR spectral data and other techniques.The anti-inflammatory activities of the compounds were determined by Griess method.Results A total of thirteen compounds were isolated from A.pierreana,and their structures were identified as 8-hydroxy-1,9-dimethoxy-[2′,2′-dimethylpyrano(5′,6′:2,3)]coumaronochromone(1),derrusin(2),scopoletin(3),caesalpiniaphenol D(4),(−)-gynuraone(5),methyl caffeate(6),ethyl caffeate(7),ethyl ferulate(8),trans-p-hydroxycinnamic acid ethyl ester(9),p-hydroxy benzoic(10),vanillic acid(11),caffeic acid(12),ferulic acid(13).Conclusion Compounds 1 was a new coumarin compound of benzofuran[3,2-c]and named as argypierstan.Compounds 2,4—6,8—13 were isolated from A.pierreana for the first time.Compounds 4,6,8 and 9 exhibited mild anti-inflammatory activity and the half maximal inhibitory concentration(IC_(50))was(43.38±0.44),(4.95±0.10),(35.96±0.31)and(42.77±1.80)μmol/L,respectively.

关 键 词：东京银背藤 苯并呋喃[3 2-c]香豆素 银背藤内酯 抗炎活性 阿魏酸乙酯 反式对羟基肉桂酸乙酯 

分 类 号：R284.1[医药卫生—中药学]