基于网络医学与等辐射分析探讨“杜仲-干姜”活性成分对神经炎症的协同抑制作用  

Synergistic inhibition of neuroinflammation by active ingredient of Eucommiae Cortex and Zingiberis Rhizoma based on network medicine and isobolographic analysis

作　　者：燕子姝 李东娜 李小锦 谭思宇 张艳军[3,4] 庄朋伟 郭虹[1] YAN Zishu;LI Dongna;LI Xiaojin;TAN Siyu;ZHANG Yanjun;ZHUANG Pengwei;GUO Hong(State Key Laboratory of Component-based Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Haihe Laboratory of Modern Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300193,China)

机构地区：[1]天津中医药大学组分中药国家重点实验室,天津301617 [2]天津中医药大学现代中医药海河实验室,天津301617 [3]天津中医药大学第一附属医院,天津300193 [4]国家中医针灸临床医学研究中心,天津300193

出　　处：《中草药》2024年第15期5166-5173,共8页Chinese Traditional and Herbal Drugs

基　　金：天津市卫生健康委员会天津市中医药重点领域科研项目(2024008)。

摘　　要：目的基于网络医学与等辐射分析法探讨“杜仲-干姜”活性成分对神经炎症的协同抑制作用。方法采用网络医学的方法,通过计算杜仲-干姜的活性成分靶点模块与神经炎症基因模块之间的距离,预测具有协同作用的2种活性成分组合。使用脂多糖(lipopolysaccharide,LPS)诱导小鼠小胶质细胞(BV-2)建立神经炎症模型,评价药物组合对一氧化氮(nitric oxide,NO)释放的影响。以NO抑制率为指标,采用等辐射分析法和统计学方法计算药物组合之间的相互作用,从而判断2种成分之间协同作用。结果使用网络医学的方法计算出2种可能具有协同关系的药物活性成分组合,即6-姜烯酚与杜仲醇、6-姜酚与杜仲醇。等辐射分析法及统计学方法对体外实验结果进行计算发现6-姜烯酚与杜仲醇1∶2配伍、6-姜酚与杜仲醇2∶1配伍在抑制小胶质细胞炎症方面均具有协同作用。结论“杜仲-干姜”活性成分组合(6-姜烯酚与杜仲醇、6-姜酚与杜仲醇)在抑制神经炎症方面具有协同作用,为中药活性成分协同性研究提供了方法学参考,为活性成分的后续深入药理机制研究提供了重要信息。Objective To exploring the synergistic inhibitory effect of active ingredients from Duzhong(Eucommiae Cortex)and Ganjiang(Zingiberis Rhizoma)on neuroinflammation based on network medicine and isobolographic analysis.Methods The combination of two active ingredients with synergistic effects were predicted by calculating the distance between the active ingredient target module of Eucommiae Cortex and Zingiberis Rhizoma and the neuroinflammatory gene module through network medicine method.Lipopolysaccharide(LPS)was used to induce BV-2 cells to establish a neuroinflammatory model and the effect of drug combination on the release of nitric oxide(NO)was evaluated.NO inhibition rate was used as an indicator,the interaction between drug combinations was calculated using equal radiation analysis and statistical methods to determine the synergistic effect between the two components.Results Two possible synergistic combinations of drug active ingredients were calculated using network medicine method,namely 6-shogaol and eucommiol,6-gingerol and eucommiol.The results of in vitro experiments were calculated using isobolographic analysis and statistical methods,and it was found that the combination of 6-shogaol and eucommiol at a ratio of 1∶2,as well as the combination of 6-gingerol and eucommiol at a ratio of 2∶1,all had a synergistic effect on inhibiting microglial inflammation.Conclusion The combination of active ingredients of Eucommiae Cortex and Zingiberis Rhizoma(6-shogaol and eucommiol,6-gingerol and eucommiol)has a synergistic effect on inhibiting neuroinflammation,providing methodological references for the study of synergistic effects of active ingredients in traditional Chinese medicine and important information for the subsequent in-depth pharmacological mechanism research of active ingredients.

关 键 词：杜仲 干姜 6-姜烯酚与杜仲醇配伍 6-姜酚与杜仲醇配伍 网络医学 等辐射分析法 协同作用 神经炎症 

分 类 号：R285.5[医药卫生—中药学]