调控甘松中倍半萜类化合物积累的转录因子分析  

Analysis of transcription factors regulating accumulation of sesquiterpenoids in Nardostachys jatamansi

作　　者：何斌 曲别阿香 李敏 罗梦婷 青贤 刘十 苏琪 李莹 陈晨 刘圆 阎新佳 李文兵 张绍山 HE Bin;QUBIE Axiang;LI Min;LUO Mengting;QING Xian;LIU Shiyi;SU Qi;LI Ying;CHENChen;LIU Yuan;YAN Xinjia;LI Wenbing;ZHANG Shaoshan(College of Pharmacy,Southwest Minzu University,Chengdu 610041,China;Sichuan Technology and Engineering Laboratory of QiangYi Medicinal Resources Protection and Utilization,Chengdu 610225,China;Key Laboratory of Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization of National Ethnic Affairs Commission,Chengdu 610225,China;Sichuan Xiangyuan Rare Chinese Medicine Technology Development Co.,Ltd.,Maerkang 624011,China)

机构地区：[1]西南民族大学药学院,四川成都610041 [2]四川省羌彝药用资源保护与利用技术工程实验室,四川成都610225 [3]青藏高原民族药用资源保护与利用国家民委重点实验室,四川成都610225 [4]四川香原珍稀中药科技开发有限公司,四川马尔康624011

出　　处：《中草药》2024年第15期5222-5229,共8页Chinese Traditional and Herbal Drugs

基　　金：四川省自然科学基金资助项目(24ZDYF1401);四川省药品检验研究院/国家药品监督管理局中成药质量评价重点实验室开放课题资助(中成药-2024-KFKT-001);中央高校基本科研业务费专项资金研究类项目(2023NYXXS082)。

摘　　要：目的筛选参与调控甘松Nardostachys jatamansi主要活性倍半萜类化合物积累的bHLH、bZIP、MYB家族转录因子。方法利用甘松全长转录组数据库注释信息获取bHLH、bZIP、MYB家族转录因子,利用WGCNA分析潜在能正向调控主要倍半萜类化合物积累的转录因子,利用实时荧光定量PCR(quantitative real-time polymeras chain reaction,qRT-PCR)进一步确认候选转录因子是否影响甘松主要活性倍半萜类化合物的积累,利用MEME在线网站分析候选bHLH、bZIP、MYB基因家族成员的保守基序。结果在甘松全长转录组数据中分别筛选出bHLH家族893个、bZIP家族416个、MYB家族436个,WGCNA分析筛选能正向调控萜类化合物生物合成的转录因子共458个,qRT-PCR确认候选转录因子的表达水平与甘松主要活性倍半萜化合物的积累水平高度正相关。结论甘松主要倍半萜类化合物的积累可能受到本研究筛选的458个转录因子的正向调控作用,可作为甘松主要药效物质生物合成调控机制研究的潜在靶基因。Objective To screen transcription factors(TFs)of bHLH,bZIP,MYB family involved in regulating the accumulation of major active sesquiterpenoids in Gansong(Nardostachys jatamansi DC.).Methods The TFs of bHLH,bZIP,and MYB families were obtained by the annotation information of the full-length transcriptome database of N.jatamansi.Weighted gene co-expression network analysis(WGCNA)was used to analyze potential TFs that can positively regulate the accumulation of major sesquiterpenoids.Using quantitative real-time polymeras chain reaction(qRT-PCR)to further confirm whether candidate TFs affect the accumulation of active sesquiterpenoids in N.jatamansi.MEME online website was used to analyze the conserved motifs of bHLH,bZIP,and MYB gene family members.Results A total of 893 members of bHLH family,416 members of bZIP family,and 436 members of MYB family were screened from the full-length transcriptome of N.jatamansi;458 TFs that can positively regulate the accumulation of sesquiterpenoids were obtained by WGCNA,and qRT-PCR confirmed that the expression levels of candidate TFs were highly positively correlated with the accumulation levels of the major active sesquiterpene compounds of N.jatamansi.Conclusion The accumulation of major sesquiterpenoids in N.jatamansi may be positively regulated by the 458 TFs screened in this study,which can be used as potential target genes for the study of the biosynthesis regulation mechanism of major sesquiterpenoids in N.jatamansi.

关 键 词：甘松 转录因子 WGCNA QRT-PCR 倍半萜类 

分 类 号：R286.12[医药卫生—中药学]