不同种质白芷适宜采收期及质量评价研究  

Quality evaluation and suitable harvest period of different germplasm of Angelicae Dahuricae Radix

作　　者：张佳旭 晏宇杭 吴清华 黄成凤[1,2] 杜金雨 黄旭龙 郑全林 裴瑾 ZHANG Jiaxu;YAN Yuhang;WU Qinghua;HUANG Chengfeng;DU Jinyu;HUANG Xulong;ZHENG Quanlin;PEI Jin(State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu 611137,China;College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Sichuan Suining Quantaitang Pharmaceutical Co.,Ltd.,Suining 629000,China)

机构地区：[1]西南特色中药资源国家重点实验室,四川成都611137 [2]成都中医药大学药学院,四川成都611137 [3]四川遂宁市全泰堂药业有限公司,四川遂宁629000

出　　处：《中草药》2024年第15期5245-5255,共11页Chinese Traditional and Herbal Drugs

基　　金：四川省科技计划重点研发项目(2020YFN0152,2023YFS0338);四川省科技厅重点研发计划(重大科技专项)社会发展领域重点研发项目(2022YFS0582);国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-D-202209);成都中医药大学杏林学者青基人才专项(QJRC2022034)。

摘　　要：目的采用指纹图谱与化学模式识别方法,以香豆素为指标,探讨不同种质白芷药材Angelicae Dahuricae Radix发育过程中有效成分的含量变化规律,评价不同种质白芷的质量,为白芷药材合理采收和质量控制提供参考和依据。方法采用HPLC法测定5个种质白芷不同发育时期香豆素类成分含量,建立不同种质白芷药材化学指纹图谱,结合相似度分析、聚类分析、主成分分析对不同种质白芷进行比较和质量评价。结果建立了不同种质白芷药材的化学指纹图谱,共标定了19个共有峰,35批白芷相似度均在0.871~0.995;在各种质白芷发育过程中,总香豆素含量呈现先增加后降低的趋势,在7月中旬达到最高;5个种质白芷不同采收期样本的主成分分析结果显示:5个种质白芷的综合评分均在7月中旬最高;5个种质白芷7月中旬样本的主成分分析的综合评分结果:川白芷>南川白芷>禹白芷>祁白芷>亳白芷,且川白芷的总香豆素含量及欧前胡素含量显著高于其余种质。结论白芷香豆素类成分组成与含量既取决于种质又受发育程度的影响,各种质白芷最佳采收期为7月中旬,以川白芷质量最佳。Objective The fingerprint and chemical pattern recognition methods were used to evaluate the quality of different germplasm of Baizhi(Angelicae Dahuricae Radix,ADR)using coumarin as the index,and the change of the content of active ingredients in the development process of different germplasm of ADR was discussed,which provided a reference and basis for the rational harvesting and quality control of ADR.Methods HPLC method was used to determine the content of coumarins in different developmental stages of five germplasm species,and the chemical fingerprints of different germplasm of ADR were established,and the similarity analysis,cluster analysis and principal component analysis were combined to compare and evaluate the quality of different germplasm of ADR.Results The chemical fingerprints of different germplasm of ADR were established,a total of 19 common peaks were calibrated,and the similarity of 35 batches was 0.871—0.995;during the development of various germplasm of ADR,the total coumarin content showed a trend of first increasing and then decreasing,reaching the highest in mid-July.The principal component analysis results of samples from different harvest stages of five germplasm of ADR showed that the comprehensive scores of the five germplasm of ADR were highest in mid-July.The principal component analysis and comprehensive scoring results of five germplasm of ADR samples in mid-July showed that Sichuan Baizhi>Nanchuan Baizhi>Yubaizhi>Qibaizhi>Bobaizhi,and the total coumarin content and imperatorin content of Sichuan Baizhi were significantly higher than those of other germplasm.Conclusion The composition and content of coumarin components were determined by both germplasm and development degree,and under the experimental conditions,the best harvest period of various species was mid-July,and the quality of Sichuan Baizhi was the best.

关 键 词：白芷药材 香豆素 种质资源 采收期 指纹图谱 欧前胡素 异欧前胡素 

分 类 号：R286.2[医药卫生—中药学]