竹叶黄酮对鸡胚接种新城疫疫苗免疫效果的影响  

Effect of Bamboo Leaf Flavonoid on Immune Efficacy of Chick Embryo Inoculation with Newcastle Disease Vaccine

作　　者：姜鑫瑞 冯贺龙 杨宏春 蒋立人 汪宏才[2] 曾哲[2] 左波[2] 张腾飞[2] 罗青平[2,4] 商雨 温国元[2] JIANG Xinrui;FENG Helong;YANG Hongchun;JIANG Liren;WANG Hongcai;ZENG Zhe;ZUO Bo;ZHANG Tengfei;LUO Qingping;SHANG Yu;WEN Guoyuan(College of Animal Science and Technology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;Hubei Provincial Key Laboratory of Livestock and Poultry Pathogen Microbiology,Key Laboratory of Prevention and Control Agents for Animal Bacteriosis Ministry of Agriculture and Rural Affairs,Institute of Animal Husbandry and Veterinary,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;College of Animal Science and Technology,Guangxi University,Nanning 530004,China;Hubei Hongshan Laboratory,Wuhan 430070,China)

机构地区：[1]华中农业大学动物科学技术学院/动物医学院,武汉430070 [2]湖北省农业科学院畜牧兽医研究所,农业农村部畜禽细菌病防治制剂创制重点实验室,畜禽病原微生物学湖北重点实验室,武汉430064 [3]广西大学动物科学技术学院,南宁530004 [4]湖北洪山实验室,武汉430070

出　　处：《中国畜牧兽医》2024年第9期4174-4181,共8页China Animal Husbandry & Veterinary Medicine

基　　金：湖北省重点研发计划(2023BBB034);湖北省自然科学基金(2021CFA018);国家自然科学基金(U23A20239、32302869);国家现代农业产业技术体系(CARS-41)。

摘　　要：【目的】探究竹叶黄酮(bamboo leaf flavonoid,BLF)作为疫苗佐剂对新城疫(ND)疫苗鸡胚接种的免疫效果影响。【方法】试验选取18胚龄SPF种蛋108枚,随机分为3组:Control组(不注射任何物质)、TS组(注射0.1 mL 103.0 EID 50/0.1 mL的新城疫病毒(Newcastle disease virus,NDV)TS09-C疫苗株)和BLF+TS组(注射0.1 mL含2%BLF和103.0 EID 50/0.1 mL的NDV TS09-C疫苗株的混合液),每组36枚种蛋。试验期间,统计种蛋的孵化率和雏鸡的存活率,在雏鸡7、14、21、28日龄时,每组随机选取3只进行屠宰,采集脾脏、法氏囊、胸腺组织,统计免疫器官指数;采集雏鸡翅下静脉血液,测定血清抗体和细胞因子水平;在雏鸡28日龄时使用NDV强毒株F48E9进行攻毒保护试验,攻毒后每天观察试验雏鸡状态,记录体重变化情况,并于攻毒后第3天每组随机选取3只雏鸡进行屠宰,取十二指肠、肺脏和气管组织并检测雏鸡不同组织病毒载量。【结果】鸡胚接种后,BLF+TS组雏鸡孵化率为100%,存活率为97.22%。与TS组相比,21~28日龄时BLF+TS组雏鸡的脾脏、法氏囊和胸腺指数以及血清中白细胞介素-6(interleukin 6,IL-6)和γ-干扰素(interferon-γ,IFN-γ)含量均显著升高(P<0.05)。攻毒试验结果显示,攻毒后BLF+TS组雏鸡存活率为100%,攻毒后2~4 d BLF+TS组雏鸡体重增长速度显著低于健康对照组(P<0.05);攻毒后3~7 d时BLF+TS雏鸡体重增长速度显著高于TS组(P<0.05)。BLF+TS组雏鸡攻毒后呼吸道和消化道组织的病毒载量显著低于TS组(P<0.05)。【结论】BLF作为免疫佐剂可安全进行鸡胚接种,而且能够帮助雏鸡建立早期免疫保护,进一步提高ND疫苗免疫效果,研究结果为开发高效鸡胚免疫疫苗佐剂提供借鉴和参考。【Objective】The aim of this experiment was to explore the effect of bamboo leaf flavonoid(BLF)as a vaccine adjuvant on the immune effect of Newcastle disease(ND)vaccine in chicken embryos.【Method】The experiment selected 10818-day embryonic SPF hatching eggs and randomly divided into 3 groups:Control group(no injection of any substance),TS group(injection of 0.1 mL 103.0 EID 50/0.1 mL of Newcastle disease virus(NDV)TS09-C vaccine strain)and BLF+TS group(injection of 0.1 mL of a mixture of 2%BLF and 103.0 EID 50/0.1 mL NDV TS09-C vaccine strain),with 36 eggs in each group.During the experiment,the hatching rate of eggs and the survival rate of chicks were calculated.When the chicks were 7,14,21 and 28 days old,3 chicks from each group were randomly selected for slaughter,then spleen,bursa of Fabricius,and thymus tissue were collected,and immune organ indexes were counted.The subwing vein blood of chicks was collected and serum antibody and cytokine levels were measured.The challenge protection test was carried out with NDV strain F48E9 at 28 days of age.After challenge,the status of the chicks was observed every day,and the weight change was recorded.On the 3rd day after the challenge,3 chicks in each group were randomly selected for slaughter.Duodenum,lung and trachea tissues were collected and viral load in different tissues of chicks were detected.【Result】After chicken embryo inoculation,the hatchability rate of chicks in BLF+TS group was 100%,and the survival rate was 97.22%.Compared with TS group,the spleen,bursa of Fabricius and thymus indices as well as the serum levels of interleukin 6(IL-6)and interferon-γ(IFN-γ)contents in BLF+TS group were significantly increased(P<0.05).The results of the challenge test showed that the survival rate of chicks in BLF+TS group after challenge was 100%.The body weight increase rate of BLF+TS group was significantly lower than that of healthy control group at 2-4 days after challenge(P<0.05).The body weight growth rate of chicks in BLF+TS group was significantl

关 键 词：鸡胚接种 竹叶黄酮 新城疫疫苗 疫苗佐剂 

分 类 号：S852.65[农业科学—基础兽医学]