光照强度对条斑紫菜栽培品系丝状体光合生理的影响  

Effect of Light Intensity on the Photosynthetic Physiology of Neopyropia Yezoensis Strain from the Yellow Sea

作　　者：陶菊红[1,2] 鱼浩 姜红霞 张涛[1,3] TAO Juhong;YU Hao;JIANG Hongxia;ZHANG Tao(School of Biology and Food Engineering,Changshu Institute of Technology,Changshu 215500;Changshu Institute of Agricultural Science,Changshu 215500;Suzhou Key Laboratory of Applied Algae,Changshu 215500,China)

机构地区：[1]常熟理工学院生物与食品工程学院,江苏常熟215500 [2]常熟市农业科学研究所,江苏常熟215500 [3]苏州应用藻类学重点实验室,江苏常熟215500

出　　处：《常熟理工学院学报》2024年第5期34-39,共6页Journal of Changshu Institute of Technology

基　　金：国家藻类产业技术体系(CARS-50);江苏现代农业(紫菜)产业技术体系(JATS[2023]472、JATS[2022]516);江苏省种业振兴“揭榜挂帅”项目“条斑紫菜新品种选育与种质创制”(JBGS[2021]140);姑苏乡土人才项目“优质经济藻类(紫菜)种质性状评价及生产示范”。

摘　　要：设定不同光照强度对条斑紫菜黄海选育品系自由丝状体进行培养,并对叶绿素荧光参数和光合色素含量进行测定.结果表明,条斑紫菜品系丝状体在光照强度为30μmol/(m^(2)·s)时具有较高的实际量子效率(ΔF/Fm′),而110μmol/(m^(2)·s)和150μmol/(m^(2)·s)光强处理组样品Yield值显著降低,且随光照时间的延长呈下降趋势.样品最大量子效率(F_(v)/F_(m))随处理光照强度的上升而显著下降.低光照强度下样品快速光曲线参数初始斜率(α)高于高光照强度处理组,而不同光照强度下样品半饱和光强(Ik)和最大相对电子传递速率(rETRmax)等未表现出明显差异.光照强度的上升使得样品光化学猝灭(qP)逐步下降,而高光照强度150μmol/(m^(2)·s)处理组样品非光化学猝灭(NPQ)显著高于其他光强处理组.光合色素藻红蛋白、藻蓝蛋白和别藻蓝蛋白含量均随光照强度的上升而降低,且150μmol/(m^(2)·s)处理组要显著低于低光强处理组,叶绿素a(Chl.a)含量的变化与藻胆蛋白相似.本研究表明条斑紫菜黄海选育品系丝状体在较低的光照强度下表现出活跃的生理状态,属适应低光照强度的种质,能够通过降低光合色素含量、协调对吸收光能的分配等途径以适应光强变化,为适宜的丝状体高效培养环境因子设定提供了基础参考.The effects of light intensity on the photosynthetic physiology of Neopyropia yezoensis strain free filaments from the Yellow Sea were studied by the measurement of photosynthetic pigment and chlorophyll fluorescence.Results showed that the strain filaments exhibited a relatively high actual quantum efficiency(ΔF/Fm′)at 30μmol/(m^(2)·s)light intensity,but decreased significantly when treated with 110μmol/(m^(2)·s)and 150μmol/(m^(2)·s)light intensities,and theΔF/Fm′also decreased with the extension of lighting time.The maximum quantum efficiency(F_(v)/F_(m))of the samples decreased significantly with the increase of the light intensity.The Initial slope of the Rapid Light Curve(α)of samples under low light intensity was higher than those of samples under high light intensities,while the difference in semi saturated light intensity(Ik)and maximum relative electron transfer rate(rETRmax)was not significant.The photochemical quenching parameter(qP)of samples decreased with the increase of light intensities,while the non photochemical quenching parameter(NPQ)of the samples under the 150μmol/(m^(2)·s)light intensity was significantly higher than those samples under other light intensities.The content of photosynthetic pigments phycoerythrin,phycocyanin,and allophycocyanin all decreased with increasing light intensities,and those pigments content of sample under 150μmol/(m^(2)·s)were significantly lower than those of the sample under 30μmol/(m^(2)·s).The change in chlorophyll a content was similar to that of phycobiliprotein.This study indicates that the N.yezoensis strain free filaments from the Yellow Sea exhibits an active physiological state under low light intensity.It is a germplasm that adapts to low light intensity.The strain can adapt to changes in light intensity by reducing the content of photosynthetic pigments and coordinating the distribution of absorbed light energy.This provides experimental data for the setting of suitable environmental factors for efficient cultivation of N.yezoensis

关 键 词：条斑紫菜 品系 光合作用 叶绿素荧光 

分 类 号：Q94[生物学—植物学] S93[农业科学—渔业资源]