高分子量壳聚糖皮芯结构微纳米纤维膜制备  

Fabrication of high molecular weight chitosan core-shell nanofibers

作　　者：房磊 刘秀明[1] 贾娇娇 蔺志浩 任燕飞 侯凯文 巩继贤[1] 扈延龄[3] FANG Lei;LIU Xiuming;JIA Jiaojiao;LIN Zhihao;REN Yanfei;HOU Kaiwen;GONG Jixian;HU Yanling(School of Textile Science and Engineering,Tiangong University,Tianjin 300387,China;College of Textiles&Clothing,Qingdao University,Qingdao,Shandong 266071,China;Traumatology Department,Affiliated Hospital of Qingdao University,Qingdao,Shandong 266000,China;Shandong Xinyue Health Technology Company,Binzhou,Shandong 256600,China)

机构地区：[1]天津工业大学纺织科学与工程学院,天津300387 [2]青岛大学纺织服装学院,山东青岛266071 [3]青岛大学附属医院创伤外科,山东青岛266000 [4]山东欣悦健康科技有限公司,山东滨州256600

出　　处：《纺织学报》2024年第9期1-9,共9页Journal of Textile Research

基　　金：天津市研究生科研创新项目(2022BKY141);山东省科技型中小企业创新能力提升工程计划项目(2021TSGC1189)。

摘　　要：高分子量壳聚糖因具有良好的抗菌性、可促进细胞和组织生长以及可降解等特性,被认为是促进伤口愈合的优良材料;然而高分子量壳聚糖纺丝液黏度大,用常规静电纺丝法难以制成微纳米纤维。为解决这个问题,采用溶液喷射纺丝法制备了高分子量壳聚糖/聚氧化乙烯微纳米纤维,纺丝液含有质量分数为1.6%的高分子量壳聚糖和2.5%~5.0%的聚氧化乙烯(相对分子质量为10万)。所制备的微纳米纤维呈直线形,表面呈不光滑波纹状。通过MatLab对扫描电子显微镜照片进行识别和计算,结果表明,当聚氧化乙烯质量分数从2.5%增加到5.0%时,纤维平均直径从133 nm增加到210 nm,直径分布变宽;微纳米纤维材料中大孔隙增多,孔隙率在0.69左右,变化不大;X射线光电子能谱和透射电子显微镜分析结果表明,所制备的高分子量壳聚糖/聚氧化乙烯喷射纺丝微纳米纤维具有皮芯结构,其中,聚氧化乙烯位于皮层,高分子量壳聚糖构成微纳米纤维的内芯;动物实验初步证明了高分子量壳聚糖皮芯结构微纳米纤维可以促进伤口愈合。Objective High molecular weight chitosan(HMCS)has many advantages when used in the field of wound management because of its antibacterial properties as well as the cell and tissue growth capabilities.However,fabricating HMCS nanofiber is challenging since the spinning solution's viscosity is extremely high.In order to solve this problem,solution blow spinning was studied and adjusted to fabricate HMCS nanofibers,and the spinning parameters were identified to fabricate polyethylene oxide(PEO)as shell and HMCS as core nanofibers,which were transformable to physical hydrogel when contacting the wound exudate for wound healing.Method The spinning solutions containing 1.6%mass fraction HMCS and 2.5%-5.0%mass fraction PEO were prepared by dissolving and mixing these two species in 50%mass fraction aqueous acetic acid solutions,with 200 r/min mixer rotation rate and 10 h mixing time.The well-mixed solutions were degassed for 12 h before solution blow spinning.In the spinning process,PEO-HMCS nanofibers were spun with the parameters ranging from 0.04 MPa to 0.10 MPa air pressures and 21 cm to 33 cm collecting distances.The area of the resulting PEO-HMCS nanofibers was 2010 cm^(2) and the spinning duration was 45 min for each of the four spinning solutions.The temperature was kept at 24℃with the relative humidity of approximately 20%during the solution blow spinning process.Results The morphologies of the PEO-HMCS nanofibers were observed by scanning electron microscopy and field emission-scanning electron microscopy.The shapes of the nanofibers were straight lines and the fiber surfaces were not smooth,with some ripple shapes.When the PEO mass fractions in solutions increased from 2.5%to 5.0%,the mean diameters of the nanofibers increased from 133 nm to 210 nm,with the nanofibers porosities anchanged and remaining at 0.69.This study also investigated the influences of changing collecting distances on the resulting nanofibers mean diameters,as well as the influences of changing air pressures on the resulting nanofibers

关 键 词：医用敷料 溶液喷射纺丝法 壳聚糖 聚氧化乙烯 微纳米纤维 皮芯结构 

分 类 号：TQ342.9[化学工程—化纤工业]