基于底物激活的蛋白酶法羊毛地毯生态丝光处理  

Eco-friendly mercerization of wool carpet using protease method based on substrate activation

作　　者：王乐 段志欣 姚金波[3] 刘建勇[3] 卢建军 WANG Le;DUAN Zhixin;YAO Jinbo;LIU Jianyong;LU Jianjun(College of Textile Engineering,Taiyuan University of Technology,Taiyuan,Shanxi 030600,China;State Key Laboratory of Clean and Efficient Coal Utilization,Taiyuan University of Technology,Taiyuan,Shanxi 030024,China;School of Textile Science and Engineering,Tiangong University,Tianjin 300387,China)

机构地区：[1]太原理工大学轻纺工程学院,山西太原030600 [2]太原理工大学省部共建煤基能源清洁高效利用国家重点实验室,山西太原030024 [3]天津工业大学纺织科学与工程学院,天津300387

出　　处：《纺织学报》2024年第9期129-136,共8页Journal of Textile Research

基　　金：山西省基础研究计划项目(20210302124459)。

摘　　要：针对蛋白酶难以降解高硫羊毛角蛋白的问题,构建了由底物激活剂LKZ-630与蛋白酶Savinase 16L组成的蛋白酶催化体系,采用该体系联合机械刮毯和刷毯对羊毛地毯进行丝光处理。通过酶活力、酶吸附性、羊毛水解度、地毯颜色、X射线衍射能谱及红外谱图等测试,分析了LKZ-630对酶水解羊毛的促进作用以及丝光处理前后羊毛地毯的外观及微观结构变化。结果表明:LKZ-630可打开羊毛纤维中二硫键,2 g/L的LKZ-630可使酶对羊毛的活力提高1575倍,可使羊毛纤维对酶的平衡吸附量提高12.7倍,使酶对羊毛的水解速率提高36.3倍;刮毯和刷毯作用使得处理后羊毛地毯中纤维细度由根部至尖端逐渐变细,处理后地毯触感柔软、有层次感且有蚕丝般光泽,其CIE亮度值增加6.99,白度值增加50.12,黄度值减小14.84。Objective In order to fundamentally solve the severe environmental pollution problem caused by the existing chlorinated wool carpet mercerizing finishing,much research has been focused on ecological mercerizing.The protease method is a mild and green finishing process.However,the low hydrolysis efficiency of protease on wool keratin remains to be a technical bottleneck,and it is necessary to develop the protease method for the wool carpet mercerizing process with ecological characteristics.It is conducive to promoting green and sustainable development of the textile industry.Method A protease-catalyzed system consisting of substrate activator LKZ-630 and protease Savinase 16L was constructed and used for mercerizing wool carpets by combining mechanical scraping and brushing.The role of LKZ-630 in the enzymatic degradation of wool was studied by measuring the sulfhydryl content on the wool surface,protease activity,protease adsorption,and degree of hydrolysis of wool.The structure of wool carpets before and after treatment was examined by scanning electron microscopy,Fourier infrared spectroscopy,and X-ray diffraction energy spectroscopy.Results The results showed that LKZ-630 could open disulfide bonds in wool fibers and convert them to sulfhydryl groups.It facilitated the rapid degradation of high-sulfur wool tissues by proteases.By adding LKZ-630,the protease activity(substrate of wool fibers)increased by 1575 times when the concentration of LKZ-630 was 2 g/L.Under the treatment conditions of LKZ-6302 g/L,Savinase 16L 1 g/L,and 50℃,LKZ-630 increased the equilibrium adsorption of protease on wool fibers by 12.7 times and increased the hydrolysis rate by 36.3 times.Wool carpet mercerizing equipment with brushing and scraping functions was developed.A protease-catalyzed system(LKZ-6302 g/L and Savinase 16L 1 g/L)combined with brushing and scraping was used for mercerizing pure wool carpets at 50℃for 40 min.Brushing and scraping cause the mechanical force on the carpet fiber to gradually increase from the tail

关 键 词：蛋白酶 羊毛 地毯 蛋白酶催化体系 丝光处理 底物激活 

分 类 号：TS131.8[轻工技术与工程—纺织材料与纺织品设计]