Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence  

作　　者：Shengyao Chen Muhammad Saqib Hafiz Sajid Khan Yuan Bai Usman Ali Ashfaq Muhammad Khalid Mansoor Abulimti Moming Jing Liu Min Zhou Saifullah Khan Niazi Qiaoli Wu Awais-Ur-Rahman Sial Shuang Tang Muhammad Hassan Sarfraz Aneela Javed Sumreen Hayat Mohsin Khurshid Iahtasham Khan Muhammad Ammar Athar Zeeshan Taj Bo Zhang Fei Deng Ali Zohaib Shu Shen 

机构地区：[1]Key Laboratory of Virology and National Virus Resource Center,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Faculty of Veterinary Science,University of Agriculture Faisalabad,Faisalabad 38040,Pakistan [4]The Children's Hospital Faisalabad,38000,Pakistan [5]Department of Bioinformatics and Biotechnology,Government College University Faisalabad,Faisalabad 38000,Pakistan f [6]Department of Microbiology,Faculty of Veterinary and Animal Sciences,The Islamia University of Bahawalpur,Bahawalpur 63100,Pakistan [7]Hubei Jiangxia Laboratory,Wuhan 430200,China [8]Key Laboratory of Special Pathogens and Biosafety,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan 430071,China [9]Armed Forces Institute of Pathology(AFIP),Rawalpindi 46000,Pakistan [10]Department of Clinical Studies,Faculty of Veterinary&Animal Sciences,Pir Mehr Ali Shah Arid Agriculture University,Rawalpindi 46300,Pakistan [11]Institute of Microbiology,Government College University Faisalabad,Faisalabad 38000,Pakistan l [12]Atta-ur-Rahman School of Applied Biosciences,National University of Science and Technology,Islamabad 44000,Pakistan [13]Department of Clinical Sciences,University of Veterinary and Animal Sciences,Lahore Sub-campus,Jhang 35200,Pakistan [14]Department of Molecular Pathology,National Medical Center DHA Phase 1,Karachi 75500,Pakistan

出　　处：《Virologica Sinica》2024年第3期369-377,共9页中国病毒学（英文版）

基　　金：supported by the National Key Research and Development Programme of China[Grant Number 2021YFC2300900,2022YFC2302700];International Partnership Programme of the Bureau of International Cooperation,Chinese Academy of Sciences[Grant Number 153B42KYSB20200013];National Natural Science Foundation of China[Grant Number U22A20363];Biological Resources Programme,Chinese Academy of Sciences[Grant Number KFJ-BRP-017-74].

摘　　要：Infectious diseases caused by arboviruses are a public health concern in Pakistan.However,studies on data prevalence and threats posed by arboviruses are limited.This study investigated the seroprevalence of arboviruses in a healthy population in Pakistan,including severe fever with thrombocytopenia syndrome virus(SFTSV),Crimean-Congo hemorrhagic fever virus(CCHFV),Tamdy virus(TAMV),and Karshi virus(KSIV)based on a newly established luciferase immunoprecipitation system(LIPS)assays,and Zika virus(ZIKV)by enzyme-linked immunosorbent assays(ELISA).Neutralizing activities against these arboviruses were further examined from the antibody positive samples.The results showed that the seroprevalence of SFTSV,CCHFV,TAMV,KSIV,and ZIKV was 17.37%,7.58%,4.41%,1.10%,and 6.48%,respectively,and neutralizing to SFTSV(1.79%),CCHFV(2.62%),and ZIKV(0.69%)were identified,as well as to the SFTSV-related Guertu virus(GTV,0.83%).Risk factors associated with the incidence of exposure and levels of antibody response were analyzed.Moreover,co-exposure to different arboviruses was demonstrated,as thirty-seven individuals were having antibodies against multiple viruses and thirteen showed neutralizing activity.Males,individuals aged40 years,and outdoor workers had a high risk of exposure to arboviruses.All these results reveal the substantial risks of infection with arboviruses in Pakistan,and indicate the threat from co-exposure to multiple arboviruses.The findings raise the need for further epidemiologic investigation in expanded regions and populations and the necessity to improve health surveillance in Pakistan.

关 键 词：ARBOVIRUS Pakistan SEROPREVALENCE Tick-borne virus Mosquito-borne virus 

分 类 号：R373.3[医药卫生—病原生物学]