Calmodulin-like 5 promotes PEDV replication by regulating late-endosome synthesis and innate immune response  

作　　者：Wen-Jun Tian Xiu-Zhong Zhang Jing Wang Jian-Feng Liu Fu-Huang Li Xiao-Jia Wang 

机构地区：[1]National Key Laboratory of Veterinary Public Health and Safety,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China [2]College of Animal Science and Technology,China Agricultural University,Beijing 100193,China [3]Beijing General Station of Animal Husbandry Service(South Section),Beijing,102218,China

出　　处：《Virologica Sinica》2024年第3期501-512,共12页中国病毒学（英文版）

基　　金：supported by the National Key R&D Program of China(2023YFD1801100);the National Natural Science Foundation of China(32172821);a CAU-Grant for the Prevention and Control of Immunosuppressive Disease in Animals(CAU-G-PCIDA)of the China Agricultural University.

摘　　要：The infection caused by porcine epidemic diarrhea virus(PEDV)is associated with high mortality in piglets worldwide.Host factors involved in the efficient replication of PEDV,however,remain largely unknown.Our recent proteomic study in the virus-host interaction network revealed a significant increase in the accumulation of CALML5(EF-hand protein calmodulin-like 5)following PEDV infection.A further study unveiled a biphasic increase of CALML5 in 2 and 12 h after viral infection.Similar trends were observed in the intestines of piglets in the early and late stages of the PEDV challenge.Moreover,CALML5 depletion reduced PEDV mRNA and protein levels,leading to a one-order-of-magnitude decrease in virus titer.At the early stage of PEDV infection,CALML5 affected the endosomal trafficking pathway by regulating the expression of endosomal sorting complex related cellular proteins.CALML5 depletion also suppressed IFN-βand IL-6 production in the PEDV-infected cells,thereby indicating its involvement in negatively regulating the innate immune response.Our study reveals the biological function of CALML5 in the virology field and offers new insights into the PEDV-host cell interaction.

关 键 词：Porcine epidemic diarrhea virus(PEDV) EF-hand protein calmodulin-like 5(CALML5) Late endosomes Innate immune response 

分 类 号：R392[医药卫生—免疫学]