ITP患者外周血Tph细胞比例及亚群分析  

Analysis of Frequencies and Subsets of Peripheral Helper T Cells in Patients with Immune Thrombocytopenia

作　　者：李伟平[1,2] 白自然[1] 田渝琴 尹春来 李霞 LI Wei-Ping;BAI Zi-Ran;TIAN Yu-Qin;YIN Chun-Lai;LI Xia(Department of Immunology,College of Basic Medical Science,Dalian Medical University,Dalian 116044,Liaoning Province,China;Department of Hematology,The Second Affiliated Hospital of Dalian Medical University,Dalian 116027,Liaoning Province,China)

机构地区：[1]大连医科大学免疫教研室,辽宁大连116044 [2]大连医科大学附属第二医院血液内科,辽宁大连116027

出　　处：《中国实验血液学杂志》2024年第5期1518-1523,共6页Journal of Experimental Hematology

基　　金：辽宁省自然基金面上项目(2021-MS-285)。

摘　　要：目的:观察免疫性血小板减少症(ITP)患者外周辅助T(Tph)细胞比例及其亚群分布情况,探讨ITP的发病机制。方法:选取2022年1月至2022年12月于大连医科大学附属第二医院就诊的25例ITP患者及25例性别年龄匹配的健康人作为对照通过流式细胞术分析外周血CD4^(+)T细胞亚群、Tph细胞比例及其亚群分布情况。结果:ITP患者外周血CD4^(+)T细胞中效应记忆T细胞比例明显高于健康对照组(P<0.05);ITP患者的Tph细胞比例亦明显高于健康对照组(P<0.001)且ITP患者Tph细胞大部分为效应记忆T细胞;Tph细胞与Tfh细胞一样表达共刺激分子ICOS和CD84;Tph细胞主要为Tph2亚型,但与健康对照组相比,ITP患者Tph1型细胞比例明显升高(P<0.05)。结论:ITP患者外周血中Tph细胞比例明显升高,尤其是Tph1型细胞比例明显高于健康对照组,Tph细胞的异常可能为ITP的发病机制之一。Objective:To investigate the frequencies and subset distribution of peripheral helper(Tph)T cells in patients with immune thrombocytopenia(ITP),and explore the pathogenesis of ITP.Methods:A total of 25 newly diagnosed ITP patients treated in The Second Affiliated Hospital of Dalian Medical University from January to December 2022 were selected,and 25 healthy volunteers(age-and sex-matched)were recruited as the control group.Flow cytometry was used to detect the subsets of CD4^(+)T cells and Tph cells.Results:The frequency of effector memory(CCR7^(-)CD45RO^(+)CD4^(+))T cells in ITP patients was significantly higher than that in healthy controls(P<0.05).The.frequency of Tph cells in ITP patients was also significantly higher than that in healthy controls(P<0.001),and most of the Tph cells in ITP patients were effector memory T cells.Furthermore,the expressions of T-cell costimulatory molecules in Tph cells,including ICOS and CD84,were similar to those in follicular helper T(Tfh)cells.CXCR3 CCR6^(-)Tph(Tph2)subgroup was dominant in Tph cells,but the frequency of CXCR3^(+)CCR6^(-)Tph(Tph1)cells in ITP patients was much higher than that in healthy controls(P<0.05).Conclusion:Tph cells,especially Tph1 cells,were abnormally expanded in ITP patients,which may be a potential etiology of ITP.

关 键 词：外周辅助T细胞 免疫性血小板减少症 CD4^(+)T细胞 

分 类 号：R558.2[医药卫生—血液循环系统疾病]