BMP-2/mBGPs/PLGA复合支架对唇腭裂骨缺损修复的体内外研究  

In vitro and in vivo studies on bone defect repair in cleft lip and palate with BMP-2/mBGPs/PLGA composite scaffolds

作　　者：高国兴 尹光福[1] 张洲 李江峰 曹舜泽 GAO Guoxing;YIN Guangfu;ZHANG Zhou;LI Jiangfeng;CAO Shunze(School of Biomedical Engineering,Sichuan University,Chengdu 610044,China)

机构地区：[1]四川大学生物医学工程学院,成都610044

出　　处：《生物医学工程研究》2024年第4期271-279,共9页Journal Of Biomedical Engineering Research

基　　金：国家重点研发计划(2018YFC1105703);四川省重点研发专项(2017SZ0047)。

摘　　要：为制备具备促进成骨和血管生成能力的人工多孔生物材料支架并探索其性能,本研究采用引入扩孔剂的水热合成法制备了大孔径介孔生物活性玻璃(mBGPs),通过真空浸渍法将骨形态发生蛋白-2(BMP-2)负载到mBGPs介孔中,随后结合溶剂浇铸粒子沥滤法成功制备了BMP-2/mBGPs与PLGA复合支架。材料表征结果表明,mBGPs呈空心球壳形式,为非晶相结构,具有无序的三维泡沫孔道结构,能持续溶出钙离子、磷酸根离子和硅酸根离子,同时mBGPs具有蛋白质负载和缓释的能力。体外实验结果表明,BMP-2/mBGPs/PLGA多孔支架利于大鼠骨髓间充质干细胞(rBMSCs)的黏附与增殖,能显著提高生物活性物质的分泌和表达。大鼠牙槽骨缺损动物模型的修复实验表明,该支架加速了新骨组织的生长速度和成熟。For the preparation of the ability to promote osteogenesis and angiogenesis artificial porous biomaterial scaffolds and explore its performance,we sythesized large-diameter mesoporous bioactive glasses(mBGPs)through hydrothermal synthesis and the incorporation of pore-expanding agents.Bone morphogenetic protein-2(BMP-2)was loaded into the mesopores of mBGPs using vacuum impregnation method.Subsequently,BMP-2/mBGPs and PLGA composite scaffolds were prepared by solvent casting particle leaching method.The material characterization results showed that mBGPs were hollow spherical shells with an amorphous phase structure and a disordered three-dimensional foam pore structure,which could continuously dissolve calcium ions,phosphate ions and silicate ions.Meanwhile,mBGPs had the ability of protein loading and slow release.The in vitro experimental results indicated that the BMP-2/mBGPs/PLGA porous scaffold enhanced the adhesion and proliferation of rat bone marrow mesenchymal stem cells(rBMSCs)and significantly increased the secretion and expression of bioactive substances.In the rat alveolar bone defect model,the scaffold accelerates the growth rate and maturation of new bone tissue.

关 键 词：唇腭裂治疗 生物活性材料 介孔材料 生长因子负载 骨组织工程支架 骨缺损修复 

分 类 号：R318[医药卫生—生物医学工程]