不同营养水平对鳜幼鱼糖异生和糖原分解的影响  

Effects of Different Nutrient Levels on Gluconeogenesis and Glycogenolysis of Juvenile Mandarin Fish Siniperca chuatsi

作　　者：马晨夕 赵金良[1] 宋银都 赵亮亮 MA Chenxi;ZHAO Jinliang;SONG Yindu;ZHAO Liangliang(Laboratory of Freshwater Fisheries Germplasm Resource,Ministry of Agriculture and Rural Affairs,Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding,National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学,农业农村部淡水水产种质资源重点实验室,水产动物遗传育种中心上海市协同创新中心,水产科学国家级实验教学示范中心,上海201306

出　　处：《水产科学》2024年第5期707-716,共10页Fisheries Science

基　　金：财政部与农业农村部国家现代农业产业技术体系建设专项(CARS-46).

摘　　要：为探究正常投喂、葡萄糖注射和饥饿3种不同营养水平对鳜内源葡萄糖生成途径的影响,测定正常投喂、葡萄糖注射和饥饿3个试验组鳜的血糖含量、肝糖原含量、胰岛素水平、胰高血糖素水平及糖异生关键酶磷酸烯醇式丙酮酸羧激酶、果糖-1,6-二磷酸酶、葡萄糖-6-磷酸酶和糖原磷酸化酶活性,以及各酶基因mRNA相对表达水平。结果显示,正常投喂组血糖、肝糖原含量均出现显著升高;胰岛素水平显著升高,胰高血糖素水平显著降低。糖异生关键酶活性无显著变化,糖原磷酸化酶活性受到抑制;果糖-1,6-二磷酸酶、糖原磷酸化酶基因表达水平受到抑制。葡萄糖注射组血糖、肝糖原含量显著升高;胰岛素水平显著升高、胰高血糖素水平显著降低;磷酸烯醇式丙酮酸羧激酶活性受到抑制,果糖-1,6-二磷酸酶、葡萄糖-6-磷酸酶活性不受抑制,糖原磷酸化酶活性受到抑制;磷酸烯醇式丙酮酸羧激酶、果糖-1,6-二磷酸酶、葡萄糖-6-磷酸酶基因表达水平无显著变化,糖原磷酸化酶基因表达水平受到抑制。饥饿5、10d,血糖水平糖原磷酸化酶活性显著升高,磷酸烯醇式丙酮酸羧激酶、果糖-1,6-二磷酸酶、葡萄糖-6-磷酸酶活性无显著变化;糖原磷酸化酶基因表达水平显著升高,磷酸烯醇式丙酮酸羧激酶、葡萄糖-6-磷酸酶基因表达水平显著升高;饥饿15d,磷酸烯醇式丙酮酸羧激酶、果糖-1,6-二磷酸酶、葡萄糖-6-磷酸酶活性显著升高,糖原磷酸化酶活性下降至饥饿前水平;磷酸烯醇式丙酮酸羧激酶、果糖-1,6-二磷酸酶、葡萄糖-6-磷酸酶基因表达水平显著升高,糖原磷酸化酶基因表达水平显著下降。结果表明,正常摄食的鳜血糖水平显著升高,糖异生途径无显著变化,糖原分解途径显著抑制。0~6h血糖升高,推测为食物消化吸收所致,6h到达“高血糖”峰值。6~16h食物消化吸收继续,葡萄糖吸收后,糖原�In order to explore the effects of three nutrient levels of normal feeding,glucose injection and starvation on the endogenous glucose production pathway of mandarin fish(Siniperca chuatsi),mandarin fish with body weight of(36.70±0.52)g were reared in cages of 1 m×1 m×1 m at stocking density of 25,25 and 15 fish per box at water temperature of(23.0±0.5)°C,respectively,and were fed live grass carp Ctenopharyngodon idella with body weight of(8.15±0.16)g fingerlings(normal feeding group),1 mg/g body weight intraperitoneal injection of glucose(glucose group)and starvation for 15 days(starvation group).Blood glucose and liver glycogen contents,insulin level,glucagon level,and activities of key enzymes in gluconeogenesis,including phosphoenolpyruvate carboxykinase(PEPCK),fructose-1,6-bisphosphatase(FBPase),and glucose-6-phosphatase(G6Pase)were measured 1,2,4,6,12 and 16 h after feeding,1,2,4,6,8,12,and 24 h after injection,and 5,10 and 15 days after starvation,and the relative mRNA expression levels was detected in each enzyme gene using real-time PCR.The results showed that blood glucose and liver glycogen contents and insulin level were significantly increased in the normal feeding group,and glucagon was significantly decreased,without significant change in the activity of key enzymes and inhibition of the activities of GPase,FBPase and GPase in gluconeogenesis.There were persistent hyperglycemia,and inhibition of PEPCK and the GPase activities and the relative expression levels of GPase in glucose injection group,without inhibition of the FBPase and G6Pase activities,and without significant changes in relative expression levels of PEPCK,FBPase,and G6Pase genes.The mandarin fish in starvation group had significant decrease in the blood glucose content in 10 days,and significant increase in glucagon content for 5 days,mainly relying on glycogenolysis to generate glucose from significantly elevated GPase activity during pre-and mid-stage(5 d and 10 d)of starvation.There were significant increase in relative expres

关 键 词：鳜 血糖水平 糖代谢途径 

分 类 号：S965.199[农业科学—水产养殖]