基于ND1基因石斑鱼属鱼类分子系统进化关系研究  

Molecular Phylogenetic Relationship in Grouper Species in Genus Epinephelus Based on Sequence of ND1 Gene

作　　者：何浩斌 张国庆 郜梓涵 胡凯红 林佳欣 周萌[1,2,3,4] 黄燕华 梁日深 HE Haobin;ZHANG Guoqing;GAO Zihan;HU Kaihong;LIN Jiaxin;ZHOU Meng;HUANG Yanhua;LIANG Rishen(Innovative Institute of Animal Healthy Breeding,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;College of Animal Science and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center,Guangzhou 510225,China;Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding,Guangzhou 510225,China)

机构地区：[1]仲恺农业工程学院健康养殖创新研究院,广东广州510225 [2]仲恺农业工程学院动物科技学院,广东广州510225 [3]广东省水环境与水产品安全工程技术研究中心,广东广州510225 [4]广州市水产病害与水禽养殖重点实验室,广东广州510225

出　　处：《水产科学》2024年第5期746-756,共11页Fisheries Science

基　　金：国家自然科学基金资助项目(31802300);清远市科技计划项目(2022KJJH064);仲恺农业工程学院研究生科技创新项目(KJCX2023028).

摘　　要：为从分子水平分析我国石斑鱼属鱼类物种分类关系,澄清分类争议,2019—2021年在广东、福建、海南、浙江、香港地区等沿海省市或地区水产市场、渔港码头,及澳大利亚采集石斑鱼属鱼类37种91尾,基于NADH脱氢酶亚基Ⅰ(ND1)基因综合分析它们的分子系统进化关系。试验结果显示:37种石斑鱼ND1基因同源序列为645bp,种间遗传距离0.0062~0.2636,平均遗传距离0.1547。以九棘鲈属为外类群构建分子系统进化树,进化树上石斑鱼属鱼类主要形成两个基本分支,大多数种类在进化树上聚类关系清晰,节点拥有较高支持率。部分形态分类存在争议的石斑鱼:吊桥石斑鱼/电纹石斑鱼、花点石斑鱼/白背石斑鱼、萤点石斑鱼/纹波石斑鱼,进化树上均聚为姐妹分支,但支长能清晰区分各物种,两两间ND1遗传距离均较大(大于2%),揭示其各自应为独立的物种。黄鳍石斑鱼与细点石斑鱼ND1遗传距离仅为0.0062,进化树支长极短,两者在分子水平可能为同一物种。试验结果展示了37种石斑鱼分子系统分类关系,澄清部分形态相似物种同种异名分类争议,可为石斑鱼属鱼类物种分类奠定分子水平分类依据。In order to analyze the taxonomic relationship of grouper species in genus Epinephelus in China at molecular level and clarify the taxonomic controversy,the molecular phylogenetic relationship was comprehensively analyzed.in 91 samples of 37 species collected in aquatic product markets,fishing ports and wharves in coastal provinces,cities or regions such as Guangdong,Fujian,Hainan,Zhejiang,Hong Kong region,and even Australia from 2019 to 2021 based on NADH dehydrogenase subunit I(ND1)gene.The result showed that the consensus ND1 gene sequences were determined to be 645 bp,with genetic distance from 0.0062 to 0.2636,and the average value of 0.1547 among species.The molecular phylogenetic trees were constructed using Cephalopholis species as outgroup,and 37 species of groupers were divided into two parallel clades Epinephelus in the tree,most species relationships with clear and high noded supports.Certain grouper pairs:E.morrhua/E.radiatus,E.maculatus/E.bilobatus,and E.coeruleopunctatus/E.ongus,with taxonomic controversies,were tightly clustered together,but the branch lengths clearly distinguished each pair of groupers.There was larger than the value 2%of genetic distances in ND1 gene in each pair of groupers,indicating that groupers in each pair were distinct species.Within the pair of groupers E.flavocaeruleus and E.cyanopodus,the ND1 genetic distance between them was 0.0062,and their branch lengths were extremely short and hard to be distinguished,revealing that they might be the same species in molecular level.The findings indicated that the molecular phylogenetic relationship among 37 species in genus Epinephulus was developed and that the synonymous controversies of certain species with similar morphological characteristics are clarified,which provides the molecular evidences for the classification of species in genus Epinephulus.

关 键 词：石斑鱼属 线粒体DNA ND1序列 系统发育 

分 类 号：Q959.4[生物学—动物学]