EM菌对蓝藻的抑制作用  

Inhibition Effect of EM Bacteria on Cyanobacteria

作　　者：周永佳 刘兴国[2] ZHOU Yongjia;LIU Xingguo(School of Life Sciences,Huzhou Normal University,Huzhou 313000,China;Fishery Machinery and Instrument Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200092,China)

机构地区：[1]湖州师范学院生命科学学院,浙江湖州313000 [2]中国水产科学研究院渔业机械仪器研究所,上海200092

出　　处：《水产科学》2024年第5期784-792,共9页Fisheries Science

基　　金：国家现代农业产业技术体系项目(CARS-46).

摘　　要：为探究EM菌对蓝藻的抑制作用,分别在蓝藻(微囊藻属含量>95%、鱼腥藻属、颤藻属、螺旋藻属)藻液中添加体积分数为0、0.40%、0.80%、1.60%、3.20%EM菌液(主要包括植物乳杆菌、热带醋杆菌和贝莱斯芽孢杆菌等),活菌总密度为1×10^(8)cfu/L,并在不同培养时间内对溶液中溶解性总氮、溶解性总磷、溶解性有机碳质量浓度,pH,蓝藻密度以及总叶绿素a质量浓度进行测定。结果表明:添加0.80%、1.60%、3.20%EM菌液可显著降低溶液中的溶解性总氮质量浓度(P<0.05),且添加量越大,溶液中的溶解性总氮质量浓度越低;前3d,EM菌液添加量为3.20%可显著降低溶液中的溶解性总磷质量浓度(P<0.05);添加EM菌液对最终溶液中溶解性有机碳质量浓度影响不大,可以稳定溶液中的pH,对蓝藻具有明显的抑制作用(P<0.01),抑制率可达90.00%,抑制效果依次为0.80%>3.20%>1.60%>0.40%;添加EM菌液可降低溶液中的总叶绿素a质量浓度,作用效果依次为0.80%>1.60%>0.40%>3.20%,其中EM菌液添加量为0.80%、1.60%对降低溶液中总叶绿素a质量浓度有显著影响(P<0.05)。综上,EM菌液添加量为0.80%对抑制蓝藻和降低总叶绿素a效果最佳,可用于控制水体中蓝藻水华。本试验结果可为微生物菌群控制蓝藻水华暴发、调控养殖水体藻相结构等提供参考。In order to evaluate the inhibitory effect of EM bacteria on cyanobacteria,EM bacteria including Lactobacillus plantarum,Acetobacter tropicalis,and Bacillus velezensis solution with total density of viable bacteria of 1×10^(8) cfu/L were added to the cyanobacteria including Microcystis(content>95%),Anabaena,Oscillatoria and Spirulina solution at a dose of 0%(control group),0.40%,0.80%,1.60%and 3.20%(volume fraction),and the concentrations of dissolved total nitrogen,dissolved total phosphorus,and dissolved organic carbon,pH,cyanobacteria density and total chlorophyll a level in the solution were determined at different culture periods.The results showed that adding 0.80%,1.60%and 3.20%EM bacterial solution led to significantly reduce the concentration of total dissolved nitrogen in solution(P<0.05).The concentration of dissolved total phosphorus in the solution was shown to be significantly decreased by adding 3.20%of EM bacterial solution in the first 3 days(P<0.05),with little effect on the concentration of dissolved organic carbon in the final solution.There was stable pH in the solution containing EM bacteria.EM liquid was found to be significant inhibitory effect on cyanobacteria(P<0.01),with inhibitory rate of up to 90.00%,and the order of inhibitory effect as 0.80%>3.20%>1.60%>0.40%.The concentration of total chlorophyll a in solution was decreased by adding EM bacterial solution,with the descending order effects of inhibitory effect were as 0.80%>1.60%>0.40%>3.20%,0.80%and significant effects on the concentration of total chlorophyll a in solution in 1.60%of EM bacterial solution(P<0.05).In summary,the best effect on inhibiting cyanobacteria and reducing total chlorophyll a was observed in 0.80%EM bacterial solution which was used to control cyanobacteria bloom in natural water or aquaculture water.The finding provides reference for controlling cyanobacteria bloom outbreak and regulating algal phase structure in aquaculture water.

关 键 词：蓝藻 EM菌 抑制率 

分 类 号：X52[环境科学与工程—环境工程]