水杨酸对鼠尾藻抗高温逆性的影响  

Effects of Salicylic Acid on High Temperature Resistance of Economic Brown Alga Sargassum thunbergii

作　　者：王晓炜 吕芳 周革非[1] 吴海一 WANG Xiaowei;LYU Fang;ZHOU Gefei;WU Haiyi(School of Life Science,YanTai University,Yantai 264005,China;Shandong Province Macroalgae Resources Conservation and Application Engineering Research Center,Marine Science Institute of Shandong Province,Qingdao 266104,China;Qingdao Key Laboratory of Coastal Ecological Restoration and Security,Qingdao 266104,China;Qingdao Macroalgae Engineering Technology Research Center,Qingdao 266104,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005 [2]山东省海洋科学研究院,山东省大型海藻资源保护与应用工程技术研究中心,山东青岛266104 [3]青岛市近海生态修复与安全保障重点实验室,山东青岛266104 [4]青岛市大型海藻工程技术研究中心,山东青岛266104

出　　处：《水产科学》2024年第5期801-807,共7页Fisheries Science

基　　金：山东省现代农业产业技术体系创新团队项目(SDAIT26);山东省农业良种工程项目(2019LZGC020).

摘　　要：为探明鼠尾藻对高温胁迫的响应以及水杨酸对其抗高温逆性的影响,将4株野生藻体置于1000mL的锥形瓶中,在15℃(对照组)下培养及30℃(处理组)下分别加入0、1、5、50mg/L水杨酸培养3d,分析高温及水杨酸对鼠尾藻的生长速率、光合色素含量、叶绿素荧光参数、抗氧化系统和热休克蛋白(HSP)70基因表达的影响。试验结果显示:水杨酸处理可显著促进高温下鼠尾藻的生长,比生长速率,叶绿素a、叶绿素c和类胡萝卜素的含量均随水杨酸质量浓度的升高先升后降,5mg/L水杨酸对生长的促进最为明显,比生长速率较高温胁迫组增加了589%,叶绿素荧光参数最大光化学量子产量有所升高,非光化学淬灭显著回升,超氧化物歧化酶和过氧化氢酶活性显著增强;HSP70基因的表达量在高温下显著升高,1mg/L水杨酸处理进一步促进了HSP70基因的表达,而5mg/L和50mg/L的水杨酸处理则抑制了高温下HSP70基因的表达。试验结果可为鼠尾藻的规模化人工繁育与抗逆机理研究提供基础数据。In order to investigate the response of brown alga Sargassum thunbergii to high temperature stress and the effect of salicylic acid on its resistance to high temperature stress,four wild alga strains were placed in a 1000 mL conical flask,and 0,1,5,and 50 mg/L salicylic acid(SA)were added at 30℃(treatment group),respectively.The growth rate,photosynthetic pigment content,chlorophyll fluorescence parameters of the alga strain were determined and the antioxidant system and HSP70 gene expression was detected after being cultured at high temperature for 3 days at 15℃(control group)and 30℃at different concentrations.The results showed that SA treatment significantly promoted the growth of the brown alga under high temperature.There were increase first and then decrease in specific growth rate,and chlorophyll a,chlorophyll c and carotenoid contents with elevated SA concentration,with the the most significant growth promotion in 5 mg/L SA group,with 589%increase in specific growth rate compared to the high temperature treatment,increase in the chlorophyll fluorescence parameter Fv/Fm,rebounded in non-photochemical quenching(NPQ)significantly,and increase in the activities of superoxide dismutase(SOD)and catalase(CAT).The expression level of HSP70 gene was found to be significantly increased under high temperature,1 mg/L SA further promoting the expression of the HSP70 gene,whereas 5 mg/L and 50 mg/L SA leading to reduce HSP70 gene expression.The findings provide theoretical reference with the study of large-scale artificial breeding and mechanisms of stress resistance in S.thunbergii.

关 键 词：鼠尾藻 水杨酸 高温胁迫 叶绿素荧光 抗氧化系统 基因表达 

分 类 号：S968.429[农业科学—水产养殖]