烟草PsaA/PsaB基因家族鉴定及其对逆境胁迫的响应  

Identification of PsaA/PsaB gene family in tobacco and its response to stress

作　　者：常永春 尹国英 燕一波 郭玉双 羽王洁 彭一洪 吴玉香[1] CHANG Yongchun;YIN Guoying;YAN Yibo;GUO Yushuang;YU Wangjie;PENG Yihong;WU Yuxiang(College of Agriculture,Shanxi Agricultural University,Taigu 030801,Shanxi,China;Guizhou Academy of Tobacco Science,Guiyang 550081,Guizhou,China;Department of Horticulture and Landscape,Zhangzhou City College,Zhangzhou 363000,Fujian,China;College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150006,Heilongjiang,China;College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China)

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]贵州省烟草科学研究院,贵州贵阳550081 [3]漳州城市职业学院园林园艺系,福建漳州363000 [4]黑龙江大学现代农业与生态环境学院,黑龙江哈尔滨150006 [5]西南大学农学与生物科技学院,重庆400715

出　　处：《陕西师范大学学报（自然科学版）》2024年第5期55-70,共16页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：中国烟草总公司贵州省公司科技项目(2022XM01);中国烟草总公司重大科技项目(110202201014(JY-14))。

摘　　要：PsaA和PsaB是光系统Ⅰ的中心蛋白,在植物光合作用及逆境调控中发挥重要功能。烟草(Nicotiana tabacum L.)是植物功能基因组学研究的模式作物,目前关于其PsaA和PsaB基因功能的研究较少。为解析烟草PsaA/PsaB基因对逆境胁迫的响应规律,在烟草基因组中鉴定PsaA/PsaB基因家族,并对其理化性质、亚细胞定位、系统进化、Motif基序、启动子调控元件进行分析。结果表明:烟草中共有27个PsaA/PsaB基因,与拟南芥相比,烟草PsaA/PsaB基因家族大量扩增,根据系统发育和结构特征可分为3类亚家族。烟草PsaA/PsaB基因启动子中含有大量响应光、低温、干旱及植物激素等的顺式作用元件。RT-qPCR分析发现,多数烟草PsaA/PsaB基因在低温胁迫下表达量上调,上调数目为8个;而在聚乙二醇、茉莉酸甲酯胁迫下表达量下调,下调数目分别为8个和7个;马铃薯Y病毒(potato virus Y,PVY)和烟草花叶病毒(tobacco mosaic virus,TMV)感染后大多数烟草PsaA/PsaB基因表达量下调,PVY感染后下叶肉和叶脉组织中基因下调数目分别为19个和17个,其中叶脉中下调更明显,TMV感染后基因下调数目为9个。可见,烟草PsaA/PsaB基因参与烟草对多种非生物胁迫的应答过程,研究结果为进一步探索PsaA/PsaB基因在调控植物对逆境胁迫响应中的作用提供了参考。PsaA and PsaB are central proteins in photosystemⅠ,and play important roles in plant photosynthesis and stress regulation.Tobacco(Nicotiana tabacum L.)is a model crop for plant functional genomics research,while there are few studies on the functions of PsaA and PsaB genes.In order to understand the response of tobacco PsaA/PsaB genes to stress,the PsaA/PsaB gene family in tobacco genome were identified,and their physicochemical properties,subcellular localization,phylogenetic evolution,motifs and promoter regulatory elements were analyzed.The results showed that there are 27 PsaA/PsaB genes in tobacco.Compared with Arabidopsis thaliana,the tobacco PsaA/PsaB gene family undergone significant expansion and can be classified into three subfamilies based on their phylogenetic and structural features.The promoters of tobacco PsaA/PsaB genes contain numerous cis-regulatory elements responsive to light,low temperature,drought and plant hormones.RT-qPCR analysis revealed that most tobacco PsaA/PsaB genes were up-regulated under low temperature stress(8 up-regulated genes)and down-regulated under polyethylene glycol and jasmonic acid methyl ester stresses(8 and 7 down-regulated genes respectively).After potato virus Y(PVY)and tobacco mosaic virus(TMV)infection,the expression of most tobacco PsaA/PsaB genes were down-regulated.The down-regulated genes in the lower mesophyll and vein tissues were 19 and 17 after PVY infection,and 9 genes were down-regulated after TMV infection.In conclusion,tobacco PsaA/PsaB genes are involved in the response process of tobacco to various abiotic stresses,and the study results provide a reference for further exploring the role of PsaA/PsaB genes in regulating plant response to stress.

关 键 词：烟草 PsaA/PsaB基因家族 聚乙二醇响应 茉莉酸甲酯响应 干旱胁迫 低温胁迫 TMV响应 PVY响应 

分 类 号：Q94[生物学—植物学]