增强型锂离子荧光探针的合成及成像应用  

Synthesis and imaging study of a fluorescent enhanced Li+fluorescent probe

作　　者：康锦 郝露 KANG Jin;HAO Lu(Department of Chemistry and Chemical Engineering,Jinzhong University,Jinzhong 030600,China)

机构地区：[1]晋中学院化学化工系,山西晋中030600

出　　处：《化学研究与应用》2024年第9期2080-2086,共7页Chemical Research and Application

基　　金：山西省基础研究计划项目(202303021211190)资助。

摘　　要：Li^(+)的检测在环境、生物及医学等研究领域具有重要意义。基于7-硝基-2,1,3-苯并氧杂噁二唑(NBD)荧光团,连接氮杂12-冠-4醚为Li^(+)识别位点,简洁高效合成了Li^(+)荧光探针(NBD-CE)。通过UV-Vis吸收光谱和荧光光谱研究了探针NBD-CE对Li^(+)的检测性能。探针NBD-CE是一种具有较好水溶性的增强型Li^(+)荧光探针,检测Li^(+)具有灵敏度高(DL=0.25μM),响应快速(2.5 s)等优点,可以用于Li^(+)的原位-实时检测。细胞毒性实验结果表明,探针NBD-CE细胞毒性较小,具有良好的生物相容性。进一步,通过荧光成像研究发现探针NBD-CE能够用于Hela细胞内Li^(+)的检测成像。Detection of lithium has great significance in environmental sciences,biology and medicine.In this work,a Li^(+)fluorescence probe(NBD-CE)is synthesized effectively by using NBD as the fluorescent group and crown ether as the Lit binding group.The detection performance of the NBD-CE for Li^(+)is studied through UV-Vis absorption spectroscopy and fluorescence spectroscopy.This probe has good water solubility.The results indicate that the NBD-CE has the advantages of high sensitivity(DL=0.25μM)and fast response(2.5 s)in detecting Li^(+).The results of cytotoxicity test shows that the probe NBD-CE has low cytotoxicity and good biocompatibility.Furthermore,this probe can be used to image of Lit in Hela cells.This study has potential application value for lithium detection in vitro and vivo.

关 键 词：荧光探针 Li^(+)检测 冠醚 荧光成像 

分 类 号：O657.3[理学—分析化学]