基于生物信息学分析探索普通登革热特征基因及免疫浸润特点  

Exploring characteristic genes and immune infiltration features of dengue based on bioinformatics

作　　者：谢铱子 王凯 詹少锋[3,4] 黄慧婷 江勇[1] 温武金 XIE Yizi;WANG Kai;ZHAN Shaofeng;HUANG Huiting;JIANG Yong;WEN Wujin(Department of Pulmonology/Respiratory and Critical Care Medicine,Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine,Shenzhen 518104;College of First Clinical Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510405;Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital,Guangzhou University of Chinese Medicine,Guangzhou 510405;Guangdong Clinical Research Academy of Chinese Medicine,Guangzhou 510405)

机构地区：[1]深圳市中西医结合医院肺病科/呼吸与危重症医学科,深圳518104 [2]广州中医药大学第一临床医学院,广州510405 [3]广州中医药大学第一附属医院呼吸与危重症医学科,广州510405 [4]广东省中医临床研究院,广州510405

出　　处：《郑州大学学报（医学版）》2024年第5期634-639,共6页Journal of Zhengzhou University(Medical Sciences)

基　　金：深圳市“医疗卫生三名工程”建设项目(SZZYSM202206013);深圳市宝安区医疗卫生科研项目(2023JD124,2023JD105);广州市科技计划项目(2023A04J1168)。

摘　　要：目的:探究普通登革热的特征基因及免疫浸润特点。方法:基于GEO数据库中的GSE96656、GSE206829芯片与R软件,分析普通登革热患者与健康者之间的差异表达基因。利用随机森林模型以及LASSO回归模型筛选疾病特征基因,并通过外部数据集验证基因表达情况。采用CIBERSORT算法进行普通登革热的免疫浸润分析,并分析特征基因与免疫细胞浸润的相关性。结果:共获得500个差异表达基因。随机森林模型以及LASSO回归模型筛选出IFI44L、FMO4作为疾病特征基因。外部数据集验证发现IFI44L在普通登革热患者全血中呈高表达。IFI44L与M1巨噬细胞、活化的树突状细胞和记忆性B细胞的浸润程度呈正相关;FMO4与M1巨噬细胞、活化的记忆性CD4^(+)T细胞的浸润程度呈正相关(P<0.05)。结论:IFI44L、FMO4可能是普通登革热的特征基因,且与免疫细胞如M1巨噬细胞浸润有关。Aim:To investigate the characteristic genes and immune infiltration features of dengue.Methods:Based on the GEO database gene chips(GSE96656 and GSE206829)and R software,differentially expressed genes(DEG)between patients with dengue and healthy people were analyzed.Characteristic genes were screened by random forest model and LASSO regression model.And the gene expressions were validated with external datasets.CIBERSORT algorithm was adopted to perform the immune cell infiltration analysis of dengue,and the correlations between characteristic genes and immune cell infiltration were also analyzed.Results:A total of 500 DEG were obtained,Random forest model and LASSO regression model pointed out IFI44L and FMO4 as characteristic genes.External gene chip validation revealed that IFI44L showed a high expression in dengue patients.IFI44L was positively correlated with the infiltration degree of M1 macrophages,activated dendritic cells and memory B cells(P<0.05).FMO4 was positively correlated with the infiltration degree of M1 macrophages and activated memory CD4^(+)T cell(P<0.05).Conclusion:IFI44L and FMO4 have the potential to serve as characteristic genes of dengue and are associated with immune cell infiltration such as M1 macrophages infiltration.

关 键 词：登革热 生物信息学 特征基因 免疫细胞浸润 

分 类 号：R512.99[医药卫生—内科学]