电针风池穴对MCAO/R大鼠运动功能及运动皮层缺血周围灶Olig2蛋白的影响  

Effects of electroacupuncture at Fengchi(GB20)on motor function and Olig2 protein around motor cortex ischemic lesion in MCAO/R rats

作　　者：陈吕佳 张英杰[2] 郝铃钰 徐鸣曙[2] CHEN Lüjia;ZHANG Yingjie;HAO Lingyu;XU Mingshu(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,China)

机构地区：[1]上海中医药大学,上海201203 [2]上海市针灸经络研究所,上海200030

出　　处：《上海针灸杂志》2024年第9期1017-1024,共8页Shanghai Journal of Acupuncture and Moxibustion

基　　金：上海市卫生健康委员会科研项目(2020LQ020);上海市科学技术委员会科研计划项目(17ZR1427500);上海市人才发展基金项目(2017094)。

摘　　要：目的探讨电针风池穴治疗脑缺血再灌注损伤的可能机制。方法将40只雄性SPF级SD大鼠随机分为空白组、空白电针组、模型组、模型电针组,每组10只。空白组不进行任何处理,空白电针组采用电针风池穴治疗,模型组进行线栓法大脑中动脉闭塞/再灌注(middle cerebral artery occlusion/reperfusion,MCAO/R)模型制备,模型电针组进行线栓法MCAO/R模型制备并采用电针风池穴治疗。4组在电针前后均进行体质量测量、Cat Walk步态检测,电针治疗结束后进行2,3,5-三苯基氯化四氮唑(2,3,5-Triphenyltetrazolium Chloride,TTC)染色观察脑梗死情况,蛋白免疫印迹(Western blot,WB)、免疫荧光检测少突胶质细胞转录因子2(oligodendrocyte transcription factor 2,Olig2)蛋白表达。结果与空白组比较,模型组大鼠脑梗死面积显著增大(P<0.01),体质量显著下降(P<0.01),四肢摆动速度明显减慢(P<0.05),左前脚掌宽度明显减小(P<0.05),WB及免疫荧光检测运动皮层区Olig2表达减少(P<0.05,P<0.01)。与模型组比较,模型电针组大鼠脑梗死面积明显减小(P<0.01),体质量增加(P<0.05),造模后2~3 d大鼠四肢摆动速度增加(P<0.05),造模后3~4 d大鼠左前脚掌宽度增大(P<0.05),WB及免疫荧光检测运动皮层缺血周围灶区域Olig2表达增加(P<0.01)。结论电针风池穴可上调再灌注后大鼠运动皮层区域缺血周围灶Olig2蛋白的表达,减小再灌注后大鼠脑组织梗死面积,促进运动皮层的修复,加速患肢平衡与稳定性的恢复。Objective To discuss the plausible mechanism of electroacupuncture at Fengchi(GB20)in treating cerebral ischemia-reperfusion injury.Method Forty male SPF-grade Sprague-Dawley(SD)rats were randomized into a blank control group,a blank electroacupuncture group,a model group,and a model electroacupuncture group,with 10 rats in each group.The blank control group received no intervention,the blank electroacupuncture group received electroacupuncture at Fengchi,the model group underwent model preparation with middle cerebral artery occlusion/reperfusion(MCAO/R),and the model electroacupuncture group received MCAO model preparation and electroacupuncture at Fengchi.The four groups of rats were weighed and observed with CatWalk gait detection before and after the electroacupuncture intervention;after the electroacupuncture intervention,cerebral infarction was observed using staining with 2,3,5-triphenyltetrazolium chloride(TTC),and the protein expression of oligodendrocyte transcription factor 2(Olig2)was detected using Western blot(WB)and immunofluorescence.Result Compared with the blank control group,the model group rats had significantly larger cerebral infarction areas(P<0.01),lower body masses(P<0.01),slower limb swing speeds(P<0.05),smaller left forepaw widths(P<0.05),and lower expression of Olig2 in the motor cortex according to the WB and immunofluorescence results(P<0.05,P<0.01).Compared with the model group,the model electroacupuncture group had significantly smaller cerebral infarction areas(P<0.01),larger body masses(P<0.05),faster limb swing speeds 2-3 d after modeling(P<0.05),larger left forepaw widths 3-4 d after modeling(P<0.05),and higher expression of Olig2 around the ischemia lesion in the motor cortex according to the WB and immunofluorescence results(P<0.01).Conclusion Electroacupuncture at Fengchi(GB20)can upregulate the expression of Olig2 protein around the ischemia lesion in the motor cortex,reduce the cerebral infarction area,boost the recovery of motor cortex,and accelerate the recovery of the

关 键 词：针刺疗法 电针 脑缺血 再灌注损伤 运动功能 少突胶质细胞转录因子2 卒中 大鼠 穴 风池 

分 类 号：R2-03[医药卫生—中医学]