二次供水系统不锈钢改造对水质微生物安全性的成效  

Effect of Stainless Steel Reconstruction on Microbial Safety of Water Quality in Secondary Water Supply System

作　　者：范晶璟 白晓慧[2] FAN Jingjing;BAI Xiaohui(Shanghai Lingang Water&Wastewater Development Co.,Ltd.,Shanghai 201305,China;School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海临港供排水发展有限公司,上海201305 [2]上海交通大学生命科学技术学院,上海200240

出　　处：《净水技术》2024年第9期136-141,共6页Water Purification Technology

基　　金：高品质饮用水智慧管理关键技术研究(沪水科2023-04)。

摘　　要：二次供水系统材质对输配水质有重要影响。为了研究二次供水系统更新不锈钢材质对管网微生物水质改善成效,采用基于R2A贫营养培养基的异养菌平皿计数(HPC)检测方法和基于16S rRNA基因高通量测序的微生物组学技术对上海市直饮水示范区不锈钢材质改造前后的微生物质量进行了评估。结果表明,不锈钢管道改造可以有效降低二次供水系统中的微生物数量。在夏季对供水末端实施不锈钢材质改造后,供水水质中HPC数量明显下降。其中,高层泵后从改造前的平均968 CFU/mL降至178 CFU/mL,降幅约达82%;6楼立管从改造前的平均1383 CFU/mL降至88 CFU/mL降幅约达93.7%。未改造小区B的HPC整体水平高于改造小区A,改造后的小区B的HPC数量低于改造前。温度对二次供水末端微生物数量影响明显,10月数量明显高于11月和12月。从原水、出厂水到泵房水池进水,优势菌属由不动杆菌属转变为假单胞菌属,入户后Phreatobacter菌属逐渐占据优势。改造前的小区B相比改造后小区A拥有潜在致病表型和生物膜形成能力表型的微生物相对丰度更高,并主要由高丰度的不动杆菌属组成。二次供水不锈钢管材更换可以通过降低有关生物膜形成功能相关和致病相关的细菌丰度来控制饮用水中的部分微生物风险。The material in secondary water supply system has a significant impact on the supplied water quality.In order to study the effect of stainless steel material renewal on the improvement of microbial water quality in the secondary water supply system,the microbiological quality of drinking water in the Shanghai Direct Drinking Water Demonstration Zone before and after stainless steel materials renovation was evaluated using the heterotrophic bacteria plate count(HPC)detection method based on R2A medium and the microbiome technology based on 16S rRNA gene high-throughput sequencing.The results showed that the renovation of stainless steel pipes can effectively decrease the microbiological number in the secondary water supply system.After the implementation of stainless steel material reconstruction at the secondary water supply system in summer,the quantity of HPC decreased greatly.Among them,the average of the high-rise pump decreased from 968 CFU/mL before the renovation to 178 CFU/mL,a decrease of about 82%.The 6th floor riser had decreased by about 93.7%from an average of 1383 CFU/mL before the renovation to 88 CFU/mL.The overall level of HPC in unmodified community B was higher than that in modified community A,and the number of HPCs in renovated community B was lower than before the renovation.Temperature could signficantly influence the HPC number in the secondary water supply system.The number in October was higher than that in November and December.From the raw water,treated water to the water inlet of the pump station,the dominant bacteria changed from Acinetobacter to Pseudomonas,and after entering the house.Phreatobacter gradually dominated.Compared with modified community A,community B before reconstruction has higher relative abundance of microorganisms with potential pathogenic phenotype and biofilm forming ability phenotype,and was mainly provided by Acinetobacter with high abundance.The replacement of stainless steel pipes in the community can control some microbial risks in drinking water by reduci

关 键 词：饮用水 不锈钢管材 异养菌平板计数 微生物组高通量测序 管网水质 

分 类 号：TU991[建筑科学—市政工程]