杂交3倍体泥鳅鳍细胞系染色体组构成研究  

Study on Chromosome Composition of Hybrid Triploid Loach ( Misgurnus anguillicaudatus) Fin Cell Line

作　　者：郭文轩 周子昱 马可馨 杨悦瑶 李逸帆 李雅娟 周贺 GUO Wen-xuan;ZHOU Zi-yu;MA Ke-xin(College of Aquatic and Life Science,Dalian Ocean University,Dalian,Liaoning 116023)

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023

出　　处：《安徽农业科学》2024年第18期67-70,77,共5页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31272650)。

摘　　要：通过天然4倍体(4n=100)和2倍体(2n=50)泥鳅杂交创制不同子代杂交组合(4n♀×2n♂、2n♀×4n♂)。以传代20次的杂交3倍体泥鳅鳍细胞系为材料,采用常规冷滴片法制备染色体标本,通过吉姆萨染色确定数目及核型;通过银染法及CMA_3/DA/DAPI三重荧光染色技术对其进行带型分析。结果显示:正反交3倍体鳍细胞系的染色体分裂相数目为3n=75,核型公式15m+6sm+54t,NF=96;正反交3倍体泥鳅鳍细胞系的染色体分裂相中显示有3个银染点,均位于第1组中部着丝粒染色体(M1)短臂的端部区域;正反交3倍体泥鳅鳍细胞的染色体中期分裂相中显示有3个CMA_3荧光信号点,均位于M1区域。研究表明:杂交3倍体泥鳅鳍细胞经细胞培养传代20次后的染色体组成未发生改变,其染色体数目、核型和带型与3倍体泥鳅其他体细胞结果一致。In this study,a natural tetraploid loach(4 n=100)was crossed with a diploid loach(2 n=50)thus creating the different offspring hybrid combinations(4n♀×2n♂,2n♀×4n♂).Chromosome samples of hybrid triploid loach fin cell passed for 20 times were prepared via conventional cold drop method.The chromosome number and karyotype were determined via Giemsa staining;Banding analysis were conducted via Ag-NORs and CMA 3/DA/DAPI staining.The results showed that the metaphase chromosome number of the fin cells of the hybrid triploid loach was 3 n=75,and the karyotype formula was 15 m+6sm+54 t,NF=96;three silver staining signal points were observed in the chromosome metaphase of hybrid triploid loach fin cells,located at the end region of the first pair of central centromeric chromosomes(M1);there were three CMA 3 signal points in the chromosome metaphase of hybrid triploid loach fin cells,which were located at the region of chromosomes(M1).The study showed that the chromosome composition of hybrid triploid loach fin cells remained unchanged after 20 times of cell culture passage,and the chromosome number,karyotype and band type were consistent with other somatic chromosomes of triploid loach.The results laid the foundation for the cytogenetics of hybrid triploid loach and provided a theoretical basis for cytogenetics of triploid loaches.

关 键 词：杂交3倍体泥鳅 鳍细胞系 核型分析 银染 CMA_3/DA/DAPI 

分 类 号：S966.4[农业科学—水产养殖]