经典名方清金化痰汤基准样品HPLC指纹图谱及量值传递研究  

Study on HPLC Fingerprint and Quantity Transfer Rule of Benchmark Sample of Qingjin Huatan Decoction

作　　者：汤瑜晨 胡云飞 沙鑫 陈伟 周桂芳 李文龙 TANG Yu-chen;HU Yun-fei;SHA Xin;CHEN Wei;ZHOU Gui-fang;LI Wen-long(College of Pharmaceutical Engineering of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Haihe Laboratory of Modern Chinese Medicine,Tianjin 301617,China;Shanghai Zhen Ren Tang Pharmaceutical Co.,Ltd,Shanghai 200000,China)

机构地区：[1]天津中医药大学中药制药工程学院,天津301617 [2]现代中医药海河实验室,天津301617 [3]上海真仁堂药业有限公司,上海200000

出　　处：《分析测试学报》2024年第9期1389-1397,共9页Journal of Instrumental Analysis

基　　金：上海市2022年度“科技创新行动计划”生物医药科技支撑专项项目(22S21904600);亳州市重点研发计划项目(bzzc2023031)。

摘　　要：基于建立的高效液相色谱(HPLC)指纹图谱结合多指标成分含量的测定方法,研究了清金化痰汤(QJHTD)的量值传递规律。制备15批QJHTD基准样品,建立其HPLC指纹图谱并进行相似度评价,确定共有峰;对QJHTD基准样品中4种指标性成分(栀子苷、芒果苷、黄芩苷、甘草酸)进行含量测定,分析饮片-基准样品的量值传递规律。结果表明,15批QJHTD基准样品指纹图谱的相似度均大于0.98。标定了26个共有峰,并对各共有峰进行药材归属。指标性成分栀子苷、芒果苷、黄芩苷、甘草酸的含量分别为44.60~76.82mg·g^(-1)、98.04~145.25 mg·g^(-1)、46.82~75.64 mg·g^(-1)、81.60~126.63 mg·g^(-1),转移率分别为32.91%~62.42%、21.78%~35.93%、29.98%~57.12%、27.51%~49.34%。15批QJHTD基准样品的出膏率为16.98%~25.19%,平均出膏转移率为67.19%。结合指纹图谱、多成分含量测定以及出膏率,研究经典名方QJHTD基准样品的量值传递规律,可为后续制剂质量控制提供参考。The HPLC fingerprint combined with a multi index component content determination method was established to study the quantity-quality tranmitting in Qingjin Huatan Decoction(QJHTD).15 batches of QJHTD substances benchmark samples were prepared,and HPLC finger⁃print spectra of QJHTD substances benchmark samples were established,evaluated the similarity and determined common peaks.A method for determining the content of four indicator components(geniposide,mangiferin,baicalin,and glycyrrhetinic acid)in the QJHTD substances benchmark samples was established,and a study was conducted on the quantity-quality tranmitting between the crude herbs and substance benchmarks.The results showed that the similarity between the fingerprint spectra of 15 batches of QJHTD substances benchmark samples and the reference spectra was greater than 0.98.26 common peaks were demarcated and the herbs were assigned to each common peak.Peaks of 10(geniposide),17,23,24 were exclusive to Zhizi(Gardeniae Fructus,GF),peaks of 11(mangiferin),12,13 were exclusive to Zhimu(Anemarrhenae Rhizoma,AR),peaks of 19(ba⁃icalin)was exclusive to Huangqin(Scutellariae Radix,SR),peaks of 25(glycyrrhetinic acid)was exclusive to Gancao(Glycyrrhiza Radix,GR).In addition,peaks of 1-4,6,9 were the common peaks of SR,Jiegeng(Platycodonis Radix,PR),Sangbaipi(Mori Cortex,MC),Beimu(Fritillari⁃ae Thunbergii Bubus,FTB),AR,Gualouzi(Trichosanthis Semen,TS),Juhong(Citri Exocarpium Rubrum,CER),Fuling(Poria,P)and GR.Peaks of 5 was the common peaks of PR,FTB,AR,TS,CER,P and GR.Peaks of 7 was the common peaks of SR,PR,Maidong(Ophiopogonis Ra⁃dix,OR),MC,FTB,TS,CER,P and GR.Peaks of 8 was the common peaks of SR,PR,OR,MC,FTB,AR,TS,CER,P and GR.Peaks of 14 and 20 were the common peaks of PR,OR,MC,FTB.Peaks of 15 was the common peaks of PR,OR,MC.Peaks of 16,21,22 were the common peaks of PR,OR,MC,FTB,TS,P and GR.Peaks of 18 was the common peaks of SR,GF,PR,OR,MC,FTB,TS,P and GR.Peaks of 26 was the common peaks of PR,MC.The index components of geniposide,mangiferin,baicalin

关 键 词：清金化痰汤 出膏率 指纹图谱 量值传递 含量测定 高效液相色谱 

分 类 号：O657.63[理学—分析化学] R284.1[理学—化学]