时分复用双光子成像系统高速数据处理研究  

Research on High-speed Data Processing of Time-division Multiplexing Two-photon Imaging System

作　　者：张美林 贾豫东[1] ZHANG Meilin;JIA Yudong(School of Instrumentation Science and Opto-electronics Engineering,Beijing Information Science and Technology University,Beijing 100192,China)

机构地区：[1]北京信息科技大学仪器科学与光电工程学院,北京100192

出　　处：《计算机测量与控制》2024年第9期290-298,306,共10页Computer Measurement &Control

摘　　要：为满足高频脉冲时分复用的双独立扫描探测双光子显微成像系统中信号解多路复用、行场同步、像素值重建的需求,对高速数据的采集处理进行了研究;基于LabVIEW FPGA,结合有限状态机、FIFO缓存和并行流水线等技术,设计了一种多功能采集处理系统,通过精确的时序控制,成功实现了高速数据的无损解复用和后续成像数据处理,并能够对共振振镜非线性运动导致的图像畸变进行校正;经过调试仿真和实验测试表明,在系统单通道采样率800 MSPS、200 MHz FPGA处理时钟下,能够对160 MHz时间交错激光脉冲产生的来自不同位置的相应荧光信号进行复杂处理;经实际应用表明,系统满足实时成像的需求,为相关领域的研究者提供了有益的参考。In order to satisfy the needs of signal demultiplexing,line-field synchronization and pixel value reconstruction in the two-photon microscopy imaging system with high-frequency pulse time-division multiplexed and dual-independent scanning detection,the acquisition and processing of high-speed data are studied;Based on LabVIEW FPGA,combined with finite state machine,FIFO cache and parallel pipeline technologies,a multifunctional acquisition and processing system is designed,which successfully achieves the lossless demultiplexing of high-speed data and subsequent imaging data processing through the precise timing control,it can correct the image distortion caused by the nonlinear motion of the resonant galvanometer;After debugging,simulation and experimental testing,with single-channel sampling rates of 800 MSPS and 200 MHz FPGA clock,the system can perform complex processing on the corresponding fluorescence signals from different locations generated by 160 MHz time-staggered laser pulses.Practical applications show that the system meets the needs of real-time imaging and provides useful references for researchers in related fields.

关 键 词：时分复用 双光子显微成像系统 高速数据 解多路复用 像素值重建 图像畸变 LabVIEW FPGA 

分 类 号：TP274[自动化与计算机技术—检测技术与自动化装置]