兴安毛连菜抗炎活性部位筛选及作用机制研究  

Research on screening of anti-inflammatory active fractions of Picris darurica Fisch.and its function mechanism

作　　者：孟令锴 李洁 高长久 张朝立 李阳 吴兆华 牛君 MENG Lingkai;LI Jie;GAO Changjiu;ZHANG Chaoli;LI Yang;WU Zhaohua;NIU Jun(Section of Teaching and Research in Natural Pharmaceutical Chemistry and Pharmacology,Department of Pharmacy,Mudanjiang Medical University,Heilongjiang,Mudanjiang 157011,China;Department of Hematology,Hongqi Hospital Affiliated to Muanjiang Medical University,Heilongjiang,Mudanjiang 157011,China;Dean’s Office,Mudanjiang Medical University,Heilongjiang,Mudanjiang 157011,China)

机构地区：[1]牡丹江医科大学药学院天然药物化学与生药学教研室,黑龙江牡丹江157011 [2]牡丹江医科大学附属红旗医院血液科,黑龙江牡丹江157011 [3]牡丹江医科大学教务处,黑龙江牡丹江157011

出　　处：《中国医药科学》2024年第17期67-70,共4页China Medicine And Pharmacy

基　　金：2021年度黑龙江省省属高等学校基本科研业务费科研项目(2021-KYYWF-0487)。

摘　　要：目的筛选兴安毛连菜抗炎作用的活性部位,初步探讨其作用机制。方法制备兴安毛连菜醇提物石油醚、乙酸乙酯、正丁醇、水不同极性部位提取物;利用脂多糖(LPS)诱导RAW264.7细胞建立体外炎症模型;利用噻唑蓝(MTT)法检测不同极性提取部位对细胞增殖活性的影响,确定安全给药范围;Griess法和酶联免疫吸附测定法(ELISA)分别检测细胞上清液中一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)的含量。结果兴安毛连菜醇提物各极性提取部位药液浓度为100、50、25μg/ml时,对细胞无明显毒性;与模型组比较,兴安毛连菜醇提物乙酸乙酯部位(高、中、低剂量组)均能使炎症因子NO、TNF-α、IL-6、IL-1β的分泌量显著降低(P<0.01),并呈现剂量依赖性;石油醚部位组、正丁醇部位组、水部位组未见明显抑制NO、TNF-α、IL-6、IL-1β的分泌。结论兴安毛连菜醇提物抗炎作用活性部位主要集中在乙酸乙酯部位,其作用机制是通过抑制NO、TNF-α、IL-6、IL-1β的分泌。Objective To screen the anti-inflammatory active fractions of Picris davurica Fisch.,so as to preliminarily investigate its function mechanism.Methods Extracts of ethanol extract of Picris davurica Fisch.with different polarity fractions of petroleum ether,ethyl acetate,n-butanol and water were prepared.RAW264.7 cells were induced by lipopolysaccharide(LPS)to establish an inflammatory model in vitro.The impacts of different polarity fractions on cell proliferation activity were detected by methyl thiazolyl tetrazolium(MTT)method,and the safe administration range was determined.Griess method and enzyme-linked immunosorbent assay(ELISA)were used to detect the contents of nitric oxide(NO),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β)in cell supernatant,respectively.Results There was no obvious toxicity to cells when the concentration of liquid medicine in each polarity extracts fraction of the ethanol extract of Picris davurica Fisch.was 100,50 and 25μg/ml.Compared with the model group,the ethyl acetate fraction(the high,medium and low dose groups)of the ethanol extract of Picris davurica Fisch.could all significantly reduce the secretion of inflammatory factors NO,TNF-α,IL-6 and IL-1β(P<0.01),and showed a dose-dependent relationship.The secretion of NO,TNF-α,IL-6 and IL-1βwas not significantly inhibited in the petroleum ether fraction group,the n-butanol fraction group and the water fraction group.Conclusion The active fraction of anti-inflammatory effect of ethanol extract of Picris davurica Fisch.is mainly concentrated in ethyl acetate fraction,and its function mechanism is by inhibiting the secretion of NO,TNF-α,IL-6 and IL-1β.

关 键 词：兴安毛连菜 抗炎 活性部位 作用机制 

分 类 号：R284.1[医药卫生—中药学]