黄连纳米明胶贴对肛瘘创面模型大鼠血清中炎症因子及CD68表达的影响  

Effect of Huanglian Nano-gelatin Paste on Expression of Inflammatory Factors and CD68 in Serum of Rats with Anal Fistula Wound

作　　者：张文秀 冯文哲 石鹏 ZHANG Wen-xiu;FENG Wen-zhe;SHI Peng(Qinghai University,Xining Qinghai 810000;Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang Shaanxi 712000)

机构地区：[1]青海大学,青海西宁810000 [2]陕西中医药大学附属医院,陕西咸阳712000

出　　处：《世界中西医结合杂志》2024年第8期1491-1495,1500,共6页World Journal of Integrated Traditional and Western Medicine

基　　金：陕西省中医药管理局项目(2021-ZZ-LC013);陕西中医药大学附属医院科研课题(2020QN008)。

摘　　要：目的通过研究黄连纳米明胶贴对于大鼠血清中炎症因子及CD68表达的影响,从而观察黄连纳米明胶贴对肛瘘大鼠模型创面愈合的作用。方法将清洁级SD雄性大鼠40只按完全随机法分为四组,空白组不予以处理,其余三组造模后分别予以相应的药物外敷治疗,模型组给予凡士林,阳性对照组给予康复新液,观察组给予黄连纳米明胶贴,治疗第3、7、14天后观察创面肉芽及创面愈合情况,并测定血清白细胞介素-6(Interleukin-6,IL-6)、白细胞介素-1β(Interleukin-1β,IL-1β)及肉芽组织中CD68的含量。结果观察组新鲜肉芽组织生成快速,成纤维细胞及新生毛细血管丰富均优于模型组和阳性对照组。术后3、7、14 d,观察组创面愈合率高于阳性对照组;术后第3、7、14天,观察组血清中IL-6,IL-1β均较模型组、阳性对照组降低,差异有统计学意义(P<0.05);观察组巨噬细胞标志物CD68的表达含量均高于模型组和阳性对照组,且随着时间呈上升趋势,组间及组内比较,差异有统计学意义(P<0.05)。结论黄连纳米明胶贴能够通过巨噬细胞的调控作用影响炎症反应的过程和调控CD68的表达以加速创面进入肉芽增生和重塑期,从而促进创面的愈合。Objective To observe the effect of Huanglian nano-gelatin paste on wound healing in a rat model of anal fistula by investigating its impact on serum inflammatory factors and CD68 expression.Methods Forty clean-grade male SD rats were randomly divided into a blank group(no treatment),a model group(treated with external application of vaseline after modeling),a positive control group(treated with external application of Kangfuxin solution after modeling),and an observation group(treated with external application of Huanglian nano-gelatin paste after modeling).On the 3 rd,7 th,and 14 th days after treatment,the formation of granulation tissue and wound healing were observed,and serum levels of interleukin-6(IL-6),interleukin-1β(IL-1β),and CD68 in granulation tissue were measured.Results The observation group exhibited faster generation of fresh granulation tissue,with more abundant fibroblasts and newly formed capillaries than the model group and the positive control group.On the 3rd,7th,and 14th days post-treatment,the wound healing rate in the observation group was higher than in the positive control group.Serum levels of IL-6 and IL-1βin the observation group were significantly lower than those in the model and positive control groups(P<0.05).The expression level of the macrophage marker CD68 in the observation group was significantly higher than in the model and positive control groups,showing an increasing trend over time,with statistically significant differences both between groups and within groups(P<0.05).Conclusion Huanglian nano-gelatin paste can promote wound healing by modulating the inflammatory response and regulating CD68 expression through the action of macrophages,thereby accelerating the wound's progression to the granulation proliferation and remodeling phase.

关 键 词：黄连纳米明胶贴 巨噬细胞 炎症因子 肛瘘术后 创面愈合 

分 类 号：R285.5[医药卫生—中药学]