4种现代炮附子与仲景炮附子异同性比较研究  

Comparative study on similarities and differences between four modern Aconiti Lateralis Radix Praeparata and Zhongjing Aconiti Lateralis Radix Praeparata

作　　者：胡云瑶 唐军 杨欣 张定堃[1] 吴京 杨明[3] 许润春[1] 贺亚男[1] HU Yunyao;TANG Jun;YANG Xin;ZHANG Dingkun;WU Jing;YANG Ming;XU Runchun;HE Yanan(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Guiyang Xinqi Microwave Industry Co.,Ltd.,Guiyang 550000,China;National Key Laboratory for the Modernization of Classical and Famous Prescriptions of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]成都中医药大学药学院,西南特色中药资源国家重点实验室,四川成都611137 [2]贵阳新奇微波工业有限责任公司,贵州贵阳550000 [3]江西中医药大学,经典名方现代中药创制全国重点实验室,江西南昌330004

出　　处：《中草药》2024年第16期5437-5447,共11页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金青年科学基金项目(82304725);四川省自然科学基金青年基金项目(2023NSFSC1777);四川省中医药管理局科学技术研究专项课题(2023MS473);四川省科技计划资助(2022YFS0429)。

摘　　要：目的对仲景炮附子Aconiti Lateralis Radix Praeparata及4种现代炮附子理化性质和毒效物质基础进行对比研究,为仲景炮附子的现代炮制生产提供依据。方法以饮片质构特征、淀粉粒形态特征、多糖含量、生物碱含量等为指标,比较4种现代炮附子与仲景炮附子的差异。结果5种炮附子表面硬度、内部硬度及韧性值均呈现炮附片2(炒白附片)>炮附片1(炒黑顺片)>炒附片>微波炮附子≈仲景炮附子;5种炮附子的淀粉红外吸收峰图谱相似,炒附片可明显观察到淀粉粒,其他4种炮附子的淀粉呈高度糊化。粗多糖得率为微波炮附子>仲景炮附子>炒附片>炮附片2≈炮附片1。仲景炮附子与4种现代炮附子的生物碱含量均存在显著性差异,双酯型生物碱总含量为仲景炮附子>微波炮附子>炒附片>炮附片2≈炮附片1;单酯型生物碱总含量为微波炮附子>炒附片>仲景炮附子>炮附片2>炮附片1。微波炮附子与仲景炮附子生物碱总煎出率相近且高于其他3种炮附子。聚类分析以上指标,结果表明仲景炮附子和微波炮附子可聚为一类,炒附片、炮附片1和炮附片2聚为一类。结论微波炮附子相较其他3种现代炮附子更符合仲景炮附子,且减毒效果更好,生产周期更短,工业化生产更简便,是仲景炮附子的现代传承与创新。Objective To carry out the comparative study on the physicochemical properties and toxic substance basis of Zhongjing Paofuzi(processed Aconiti Lateralis Radix Praeparata)and four modern Paofuzi,so as to provide a basis for the modern processing and production of Zhongjing Paofuzi.Methods The differences between four modern Paofuzi and Zhongjing Paofuzi based on their texture characteristics,starch granule morphology,polysaccharide content,alkaloid content,and other indicators were compared.Results The surface hardness,internal hardness,and internal toughness all showed that Paofupian 2(stir fried Baifupian)>Paofupian 1(stir fried Heishunpian)>Chaofupian(stir fried Fupian)>microwave Paofuzi≈Zhongjing Paofuzi;The infrared absorption peaks of starch in the five types of Paofuzi were similar,but starch granules could be clearly observed in Chaofupian,while the starch in the other four types of Paofuzi showed a high degree of gelatinization.The yield of crude polysaccharides was in the sequence of microwave Paofuzi>Zhongjing Paofuzi>Chaofupian>Paofupian 2≈Paofupian 1.There was a significant difference in the alkaloid content between Zhongjing Paofuzi and four modern Paofuzi;The total content of diester type alkaloids was Zhongjing Paofuzi>microwave Paofuzi>Chaofupian>Paofupian 2≈Paofupian 1;The total content of monoester type alkaloids was microwave Paofuzi>Chaofuzi>Zhongjing Paofuzi>Paofupian 2>Paofupian 1.The total extraction rate of alkaloids from microwave Paofuzi and Zhongjing Paofuzi was similar,but higher than the other three types of Paofuzi.Cluster analysis was used to comprehensively analyze the above indicators,and the results showed that the five types of Paofuzi can be divided into two categories.Among them,Zhongjing Paofuzi and microwave Paofuzi were clustered into one category,while Chaofupian,Paofupian 1 and Paofupian 2 were clustered into one category.Conclusion Compared with the other three modern types of Paofuzi,microwave Paofuzi is more in line with Zhongjing Paofuzi,with better detoxificat

关 键 词：仲景炮附子 炮附片 质量评价 微波炮附子 炒附片 质构特征 淀粉 多糖 生物碱 红外光谱 

分 类 号：R283.6[医药卫生—中药学]