黄芩苷长循环脂质体的制备及其抗肝癌的体外评价  

Preparation of Baicalin Long-Circulating Liposomes and Their in Vitro Evaluation for Anti-Liver Lancer Activity

作　　者：汪洁 顾雪梅 吕志阳 陈璟 WANG Jie;GU Xuemei;LV Zhiyang;CHEN Jing(Nanjing University of Chinese Medicine Hanlin College,Taizhou 225300,China)

机构地区：[1]南京中医药大学翰林学院,江苏泰州225300

出　　处：《生物化工》2024年第4期58-61,67,共5页Biological Chemical Engineering

基　　金：2023年江苏省大学生创新创业项目(202313981004Y,202313981011Y);泰州市科技支撑项目(TS202232,TS202325)。

摘　　要：目的:探索黄芩苷长循环脂质体的最佳处方工艺,并考察其体外抗肝癌作用。方法:采用薄膜分散法制备黄芩苷长循环脂质体,以包封率为评价指标,通过单因素和Box-Behnken响应面试验,确定脂质体的最佳制备工艺,并考察其体外释放度行为。通过开展细胞CCK-8试验,评价黄芩苷长循环脂质体抗肝癌作用。结果:黄芩苷长循环脂质体的最优制备工艺为大豆卵磷脂用量0.045 4 g、超声破碎时间40 s、水化介质体积7.9 mL、水化温度40℃,该工艺下脂质体的实际包封率为89.02%,平均粒径为154.6 nm,其体外释放具有缓释性,对肝癌细胞具有显著抑制作用。结论:优化得到的黄芩苷长循环脂质体具有缓慢释药行为和抑制肝癌细胞增殖的作用。Objective:To explore the optimal formulation process of baicalin long-circulating liposomes and to investigate its anti-liver cancer effect in vitro.Methods:The long-cycle liposomes of baicalin are prepared by thin film dispersion method,and the encapsulation rate is used as the evaluation index,and the optimal preparation process of liposomes is determined by single factor and Box-Behnken response surface experiments,and its release behavior in vitro is investigated.The anti-liver effect of baicalin long-circulating liposomes is evaluated by carrying out cellular CCK-8 test.Results:The optimal preparation process of baicalin long-cycle liposomes is as follows:soybean lecithin dosage of 0.045 4 g,ultrasonic crushing time of 40 s,volume of hydration medium 7.9 mL,hydration temperature of 40 ℃.Under this process,the encapsulation efficiency of liposomes is 89.02%,the average particle size is 154.6 nm,and its in vitro release has sustained release and significantly inhibited hepatocellular carcinoma cells.Conclusion:The optimized baicalin long-cycle liposomes have a slow-release behavior and inhibit the proliferation of hepatocellular carcinoma cells.

关 键 词：黄芩苷 长循环脂质体 体外释放度 肝癌 

分 类 号：R285.5[医药卫生—中药学]