千金藤碱对CIA模型小鼠安全性评价  

Evaluation of the Safety of Stephanine in CIA Model Mice

作　　者：段美艳 李今 姜哲 尹英琪 刘提提[1,3] 向泽敏 徐欢欢 DUAN Meiyan;LI Jin;JIANG Zhe;YIN Yingqi;LIU Titi;XIANG Zemin;XU Huanhuan(Key Laboratory of Pu’er Tea Science of Ministry of Education,Yunnan Agricultural University,Kunming 650201,China;College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;College of Science,Yunnan Agricultural University,Kunming 650201,China;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Kunming 650201,China)

机构地区：[1]云南农业大学普洱茶学教育部重点实验室,云南昆明650201 [2]云南农业大学食品科学技术学院,云南昆明650201 [3]云南农业大学理学院,云南昆明650201 [4]云南生物资源保护与利用国家重点实验室,云南昆明650201

出　　处：《生物化工》2024年第4期97-100,共4页Biological Chemical Engineering

基　　金：云南省“兴滇英才支持计划”(XDYC-QNRC-2023-0427);云南省科技厅科技计划项目(202201AU070183,202101AU070086,202201AT070267);云南省农业基础研究联合专项(202101BD070001-034,202101BD070001-049)。

摘　　要：为评价千金藤碱对胶原诱导关节炎(CIA)模型小鼠体质量、肝脏与肾脏的影响,造模后对小鼠分别给药1.5 mg/kg阿达木单抗(阳性组)或2 mg/kg千金藤碱(给药组),监测体质量、肝脏质量及肾脏质量,H&E染色观察组织形态。实验结果表明,2 mg/kg的千金藤碱对CIA模型小鼠体质量无显著性影响,且对小鼠肝脏及肾脏无毒性。2 mg/kg千金藤碱对CIA小鼠体质量未产生影响,且未引起CIA小鼠肝脏、肾脏的病理变化,属于安全剂量。In order to evaluate the toxicity of stephanine in collagen-induced arthritis mice body weight,liver and kidney,after modeling,the mice are administered 1.5 mg/kg adalimumab(positive group) or 2 mg/kg stephanine(drug group),monitor the body weight,liver weight and kidney weight,and histological morphology is observed by H&E staining.The experimental results show that 2 mg/kg stephanine has no significant effect on the body weight of CIA model mice,and has no toxicity to the liver and kidney.2 mg/kg stephanine has no effect on the body weight of CIA mice,and does not cause pathological changes in the liver and kidney of CIA mice,which is a safe dose.

关 键 词：千金藤碱 体质量 肝脏 肾脏 

分 类 号：Q946.8[生物学—植物学]