一测多评法同时测定车前草中4种活性成分的含量  

Simultaneous Determination of the Content of Four Active Components in Plantago asiatica L.by QAMS Method

作　　者：程海英 李墨涵 赵佩 杨冰清 张存彦 CHENG Haiying;LI Mohan;ZHAO Pei;YANG Bingqing;ZHANG Cunyan(Medical College,Tianshi College,Tianjin 301700,China)

机构地区：[1]天津天狮学院医学院,天津301700

出　　处：《生物化工》2024年第4期113-117,共5页Biological Chemical Engineering

基　　金：教育部供需对接就业育人项目(2023122758605);天津市教委科研计划项目(2023KJ286)。

摘　　要：目的:建立一测多评法(Quantitative Analysis of Multi-Components by Single Marker,QAMS)同时测定车前草中原儿茶酸、儿茶素、槲皮素和木犀草素的含量,并验证此方法的可行性及适应性。方法:以槲皮素为内标物,建立其与其他3种成分间的相对校正因子,并计算4种成分含量,实现一测多评。结果:槲皮素与其他3种成分的相对校正因子分别为1.199 2、0.861 3、1.606 9;采用一测多评法测定的10批次车前草中4种活性成分的含量与外标法测定结果无显著差异。结论:该方法准确稳定,可用于车前草多指标质量控制。Objective:To establish Quantitative Analysis of Multi-Components by Single Marker(QAMS) for simultaneous determination of protocatechuic acid,catechin,quercetin and luteolin in Plantago asiatica L.,and to verify the feasibility and adaptability of this method.Methods:Using quercetin as internal standard,the relative correction factors between quercetin and other three components are established,and the contents of four components are calculated to achieve one measurement and multiple evaluation.Results:The relative correction factors of quercetin and the other 3 components are 1.199 2,0.861 3 and 1.606 9,respectively.The contents of 4 components in 10 batches of Plantago asiatica L.by QAMS had no significant difference with the results of external standard method.Conclusion:The method is accurate and stable,and can be used for multi-index quality control of Plantago asiatica L..

关 键 词：一测多评(QAMS) 车前草 相对校正因子 外标法 

分 类 号：R282.71[医药卫生—中药学]