南海海绵Callyspongia fallax化学成分的研究  

Studies on the Chemical Constituents of Sponge Callyspongia fallax from South China Sea

作　　者：吴佩熙 时川玲 吴小云 张佩丽 李骏 周乐 梁咏倩 Wu Peixi;Shi Chuanling;Wu Xiaoyun;Zhang Peili;Li Jun;Zhou Le;Liang Yongqian(School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;College of Light Industry and Food Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,Guangdong Key Laboratory of Marine Materia Medica,RNAM Center for Marine Microbiology,Chinese Academy of Sceinces,South China Sea Institute of Oceanology,Guangzhou 510301,China)

机构地区：[1]广东药科大学药学院,广东广州510006 [2]仲恺农业工程学院轻工食品学院,广东广州510225 [3]中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室,广东省海洋药物重点实验室,中国科学院海洋微生物研究中心,广东广州510301

出　　处：《广东化工》2024年第17期40-42,共3页Guangdong Chemical Industry

基　　金：广州市基础与应用基础研究项目(202201010519,202201010336,2023A04J1162);广东省海洋药物重点实验室开放课题(LMM2021-6);广东省普通高校青年创新人才项目(2021KQNCX036);大学生创新创业训练计划项目(S202010573027)。

摘　　要：对南海海绵Callyspongia fallax进行次级代谢产物的研究。采用多种色谱分离技术对95%乙醇粗提物进行分离纯化,利用核磁共振(NMR)和质谱(MS)等波谱学手段,与报道的数据进行对比,鉴定得到7个化合物,分别是(2S,3R,4E)-N-palmitoyl-octadecasphinga-4-ene(1);3,6-二苯甲基哌嗪-2,5-二酮(2);β-胆甾醇(3);胸腺嘧啶(4);尿嘧啶核苷(5);脱氧尿嘧啶核苷(6);脱氧胸腺嘧啶核苷(7)。对得到的化合物采用MTT法评价其细胞毒活性,化合物1-7均没有表现出明显细胞毒活性。化合物1和2是首次从该种属海绵中分离得到,该工作的开展丰富了美丽属海绵化合物种类多样性。Studies on the chemical constituents of sponge Callyspongia fallax from south China sea.The crude extract of sponges with 95%ethanol was separated and purified by several chromatographic separation technology.The structure of the compounds was identified by the means of NMR and MS as well as by comparison with the data reported in literature.Seven compounds were isolated from the sponge Callyspongia fallax.Those compounds were identified as(2S,3R,4E)-N-palmitoyl-octadecasphinga-4-ene(1),3,6-diphenylmethylpiperazine-2,5-dione(2),β-cholesterol(3),thymine(4),uridine(5),2'-deoxyuridine(6)and 2'-deoxythymidine(7).The biological activity was evaluated by MTT method.None of which exhibited significant cytotoxic activity.Compounds 1 and 2 werefirst isolated from this genus sponge.The implementation of this work has enriched the diversity of compounds in this genus sponge.

关 键 词：海绵 Callyspongia fallax 次级代谢产物 细胞毒活性 

分 类 号：R931.77[医药卫生—生药学]