基于转录组测序数据的许兰毛癣菌热应激相关信号通路及候选基因的筛选  

Screening of heat stress-related signaling pathways and candidate genes in Trichophyton schoenleinii based on transcriptome sequencing data

作　　者：凯迪丽亚·艾米拉江 帕丽达·阿布利孜[1] 王晓东[1] KAIDILIYA Aimilajiang;PALIDA Abulizi;WANG Xiaodong(Department of Dermatology,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学第一附属医院,乌鲁木齐830000

出　　处：《中国真菌学杂志》2024年第4期325-330,共6页Chinese Journal of Mycology

基　　金：国家自然科学基金(81960366);自治区重点研发计划项目(2021B03001-3);国家重点研发计划课题(2022YFC2504802)。

摘　　要：目的为了筛选和鉴定许兰毛癣菌热应激相关差异表达基因,进一步探索许兰毛癣菌差异表达基因的分子机制。方法本研究采用Illumina平台PE150测序策略,对热应激(42℃)、生理温度(37℃)处理30 min和常温生长(27℃)条件下的许兰毛癣菌转录组测序和差异表达基因,通过生物信息学工具进行功能注释、分类和分析相关信号通路等。结果常温组、37℃和42℃热处理样品的RNA中30076菌株分别获得146351442、142326366和134764862条原始reads。对30079菌株分别获得143815046、134081574和117121246条原始reads。对于差异表达基因进行分析结果示,差异表达基因主要富集在“细胞酰胺代谢过程”(13个上调基因和3个下调基因)、“肽生物合成过程”(12个上调基因和3个下调基因)、“核糖体”(11个上调基因)亚类(校正P<0.05)。KEGG数据库富集分析显示,参与蛋白质和碳水化合物代谢的基因以及热休克蛋白编码基因发生了显著变化,表明热应激对这些通路的影响很大。结论本研究中获得的差异表达基因,特别是与碳代谢、氨基酸的生物合成、内质网中的蛋白质加工、次生代谢物的生物合成等代谢途径相关的基因尤其是编码热休克蛋白的相关基因可能参与了许兰毛癣菌在热应激条件下的致病机制,其具体调控机制有待进一步研究。Objective In order to screen and identify the differentially expressed genes related to heat stress in Trichophyton schoenleinii(T.schoenleinii),the molecular mechanism of the differentially expressed genes in T.schoenleinii was further explored.Methods This study adopts the Illumina platform PE150 sequencing strategy for heat stress(42℃),physiological temperature(37℃)treatment for 30 minutes and under normal temperature growth(27℃)under the condition of T.schoenleinii transcriptome sequencing and differentially expressed genes by bioinformatics tools of functional annotation,classification and analysis of the related signal pathways involved,etc.Results 146351442,142326366 and 134764862 original reads were obtained from 30076 RNA strains in the normal temperature group,37℃and 42℃heat treated samples,respectively.143815046,134081574 and 117121246 original reads were obtained from strain 30079,respectively.The analysis of differentially expressed genes showed that DEGs was mainly concentrated in the subcategories of"cellular amide metabolism"(13 up-regulated genes and 3 down-regulated genes),"peptide biosynthesis"(12 up-regulated genes and 3 down-regulated genes),and"ribosome"(11 up-regulated genes)(corrected P<0.05).KEGG database enrichment analysis revealed significant changes in genes involved in protein and carbohydrate metabolism,as well as genes encoding heat shock proteins,suggesting that heat stress had a strong impact on these pathways.Conclusion The different expression genes obtained in this study,especially the genes related to carbon metabolism,amino acid biosynthesis,protein processing in endoplasmic reticulum,biosynthesis of secondary metabolites and other metabolic pathways,especially the genes encoding heat shock proteins,may be involved in the pathogenic mechanism of Trichophyton schoenleinii under heat stress conditions,and the specific regulatory mechanisms need to be further studied.

关 键 词：许兰毛癣菌 转录组 差异表达基因 信号通路 

分 类 号：R379.2[医药卫生—病原生物学]