野鸦椿果实乙酸乙酯萃取物化学成分及对HepG2细胞抑制作用研究  

Study on the chemical constituents from ethyl acetate extracts of Euscaphis japonica fruits and their inhibitory effect on HepG2 cells

作　　者：康钦钊 叶章楷 曾佑琴[3] 谭玉柱[1,4] 陈胡兰 KANG Qin-zhao;YE Zhang-kai;ZENG You-qin;TAN Yu-zhu;CHEN Hu-lan(College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Xinjiang Normal University,Urumqi 830000,China;College of Medical Technology,Chengdu University of Traditional Chinese Medicine;State Key Laboratory of Southwestern Chinese Medicine Resources,College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院,成都611137 [2]新疆师范大学,乌鲁木齐830000 [3]成都中医药大学医学技术学院 [4]西南特色中药资源国家重点实验室,成都611137

出　　处：《天然产物研究与开发》2024年第9期1472-1483,共12页Natural Product Research and Development

基　　金：厅局级项目-重庆市食品药品检验检测研究院(322019047);“杏林学者”学科人才科研提升计划-青基人才专项(330022010)。

摘　　要：研究野鸦椿(Euscaphis japonica(Thunb.)Dippel.)干燥成熟果实的乙酸乙酯部位化学成分及对HepG2细胞的抑制作用。采用多种柱色谱技术和半制备HPLC分离技术,对野鸦椿果实的乙酸乙酯部分进行分离纯化,分离所得化合物运用核磁共振技术进行结构鉴定。并采用HepG2细胞模型对这些化合物的抗肿瘤作用进行探究。最终分离得到17个化合物,分别鉴定为齐墩果酸(1)、6β-羟基白桦脂酸(2)、木鳖子酸(3)、2-氧代坡模酸(4)、坡模酸(5)、熊果酸(6)、23-醛坡模酸(7)、1-氧泰国树脂酸(8)、annurcoic acid(9)、马斯里酸(10)、山柰酚(11)、柚皮素(12)、芹菜素(13)、木犀草素(14)、圣草酚(15)、没食子酸甲酯(16)、原儿茶酸(17)。其中,化合物2、9、12~15为首次从野鸦椿属中分离得到。化合物2、5、6、10、13对HepG2细胞有抑制作用。化合物5可抑制HepG2细胞增殖和迁移,阻滞细胞周期于G1期;促进细胞内活性氧的积累,降低线粒体膜电位,促进细胞凋亡。This study aims to investigate the chemical constituents of ethyl acetate extracts of Euscaphis japonica dry and mature fruits and their inhibition effect on HeG2 cells.The ethyl acetate part of E.japonica fruits was purified by various column chromatography and semi-prepared HPLC.The isolated compounds were identified by NMR technology.The HepG2 cell model was used to explore the anti-tumor effects of these compounds.Seventeen compounds were isolated and identified as oleanolic acid(1),6β-hydroxybetulinic acid(2),momordic acid(3),2-oxopomolic acid(4),pomolic acid(5),ursolic acid(6),23-aldehydepolomic acid(7),1-oxosiaresinolic acid(8),annurcoic acid(9),maslinic acid(10),kaempferol(11),naringenin(12),apigenin(13),luteolin(14),eriodictyol(15),methyl gallate(16),protocatechuic acid(17).Among them,compounds 2,9,12-15 were first isolated from the genus.Compounds 2,5,6,10,13 had an inhibitory effect on HepG2 cells.Compound 5 inhibited HepG2 cell proliferation and migration,blocked the cell cycle in G1 phase;promoted the accumulation of reactive oxygen species,reduced the mitochondrial membrane potential,and promoted apoptosis.

关 键 词：野鸦椿 化学成分 三萜 结构鉴定 HepG2细胞 细胞凋亡 

分 类 号：R932[医药卫生—生药学]