南海冷泉沉积物来源真菌Penicillium guanacastense HDN20-0005次级代谢产物研究  

Secondary metabolites of South China Sea cold seep sediment-derived fungus Penicillium guanacastense HDN20-0005

作　　者：钱亚 王有杰 郭铠溶 朱天骄[1] 张国建[1,2] 车茜 王文雪[1] 李德海 QIAN Ya;WANG Youjie;GUO Kairong;ZHU Tianjiao;ZHANG Guojian;CHE Qian;WANG Wenxue;LI Dehai(Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Drugs and Bioproducts,Qingdao Marine Science and Technology Center,Qingdao 266237,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003 [2]青岛海洋科技中心海洋药物和生物制品功能实验室,山东青岛266237

出　　处：《中国海洋药物》2024年第4期12-18,共7页Chinese Journal of Marine Drugs

基　　金：山东省自然科学基金重大基础研究项目(ZR2021ZD28);海南省科技计划三亚崖州湾科技城科技创新联合项目(2021CXLH0012)资助。

摘　　要：目的研究南海冷泉来源真菌Penicillium guanacastense HDN20-0005的次级代谢产物。方法优化菌株培养条件,运用硅胶柱层析、ODS反相柱层析和半制备高效液相色谱法(HPLC)对其发酵产物进行分离提纯,通过质谱(MS)、核磁共振波谱(NMR)、计算ECD、DP4+分析及和已报道文献比对,对化合物结构进行解析,并进行抑菌活性评价。结果从该菌株中分离得到1个新的酸酐类化合物(R,Z)-3-ethyl-6-methyl-3,4-dihydro-2H-1,4-oxazocine-2,5,8-trione(1),1个新的色酮衍生物(E)-6,7-dihydroxy-3-(3-oxobut-1-en-1-yl)-4H-chromen-4-one(2),1个已知色酮衍生物penialidin F(3)和1个已知化合物pencolide(4)。评价了化合物1~4对7种真菌和细菌的抑制活性,结果表明化合物均无抑菌活性。此外,评价了新化合物1和2的黄嘌呤氧化酶抑制活性,结果显示化合物2具有微弱的黄嘌呤氧化酶抑制活性,抑制率为(13.16±1.57)%。Objective To study the secondary metabolites of South China Sea cold seep sediment-derived fungus Penicillium guanacastense HDN20-0005.Methods The culture conditions of the strain were optimized,and the fermented products were isolated and purified by silica gel column chromatography,ODS reverse phase column chromatography,and semi-preparative high-performance liquid chromatography(HPLC).The structures of compounds were analyzed by mass spectrometry(MS),nuclear magnetic resonance spectroscopy(NMR),calculation of ECD,DP4+analysis,and comparison with reported literature.The antimicrobial activity of the compounds was evaluated.Results One new anhydride compound,(R,Z)-3-ethyl-6-methyl-3,4-dihydro-2H-1,4-oxazocine-2,5,8-trione(1),one new chromone derivative,(E)-6,7-dihydroxy-3-(3-oxobut-1-en-1-yl)-4H-chromen-4-one(2),one known chromone derivative,Penialidin F(3),and one known compound,pencolide(4),were isolated from the strain.The antimicrobial activity of compounds 1-4 against seven fungi and bacteria was evaluated,and the results showed that all compounds had no antimicrobial activity.In addition,the inhibitory activity of xanthine oxidase was evaluated for the new compounds 1 and 2,and the results showed that compound 2 exhibited weak inhibitory activity against xanthine oxidase with an inhibition rate of(13.16±1.57)%.

关 键 词：中国南海 冷泉 真菌 次级代谢产物 

分 类 号：R931[医药卫生—生药学]