积雪草酸调控白细胞介素-6/信号转导子和转录激活子3/核转录因子-κB通路对哮喘小鼠Treg/Th17免疫平衡的影响  

Effects of Asiatic Acid on Treg/Th17 Immune Balance in Asthmatic Mice by Regulating Interleukin-6/Signal Transductor and Transcription Activator 3/Nuclear Transcription Factor-κB Pathway

作　　者：彭显 邹敏 杨宇斌 Peng Xian;Zou Min;Yang Yubin(Jingzhou Hospital of Traditional Chinese Medicine,Hubei Jingzhou 434000,China;Guangxi International Zhuang Medicine Hospital,Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530201,China)

机构地区：[1]荆州市中医医院,湖北荆州434000 [2]广西国际壮医医院,广西中医药大学附属国际壮医医院,南宁530201

出　　处：《儿科药学杂志》2024年第10期5-10,共6页Journal of Pediatric Pharmacy

基　　金：广西壮族自治区中医药管理局自筹经费科研课题,编号20210036;2022年广西国际壮医医院“青苗工程”培育项目“青年拔尖人才”项目。

摘　　要：目的:探讨积雪草酸调控白细胞介素(IL)-6/信号转导子和转录激活子3(STAT3)/核转录因子-κB(NF-κB)通路对哮喘小鼠Treg/Th17免疫平衡的影响。方法:采用卵清蛋白(OVA)致敏和激发来制备哮喘小鼠模型,随机分为模型组、积雪草酸低剂量组(54 mg/kg)、积雪草酸高剂量组(108 mg/kg)、积雪草酸高剂量(108 mg/kg)+Colivelin(IL-6/STAT3/NF-κB激活剂,2 mg/kg)组,每组各10只。另选10只小鼠在致敏和激发阶段给予等剂量生理盐水设为对照组,用积雪草酸和Colivelin分组处理各组小鼠后检测其肺组织病理形态,肺泡灌洗液(BALF)中炎性细胞计数,外周血Treg/Th17,BALF和血清中Treg、Th17细胞分泌因子水平,肺组织IL-6/STAT3/NF-κB通路相关蛋白表达。结果:与对照组比较,模型组小鼠肺组织发生明显病理损伤,外周血Treg细胞占比和Treg/Th17,BALF和血清IL-10、IL-35水平降低(P<0.05)。肺部炎症评分,BALF中白细胞计数与嗜酸粒细胞计数,外周血Th17细胞占比,BALF和血清IL-17、IL-6水平,肺组织IL-6蛋白表达与p-STAT3/STAT3、p-NF-κB p65/NF-κB p65升高(P<0.05)。低、高剂量积雪草酸可逆转哮喘模型小鼠上述病理变化,且高剂量积雪草酸作用更强。Colivelin可减弱积雪草酸对哮喘模型小鼠上述病理变化的作用。结论:积雪草酸可通过减弱IL-6表达与STAT3、NF-κB的磷酸化,抑制哮喘小鼠炎症细胞及炎症因子产生,从而减轻气道炎症及肺组织损伤,改善肺功能。Objective:To probe into the effects of asiatic acid on Treg/Th17 immune balance in asthmatic mice by regulating interleukin-6(IL-6)/signal transducer and activator of transcription-3(STAT3)/nuclear transcription factor-κB(NF-κB)pathway.Methods:Asthmatic mice models were prepared by sensitization and stimulation with ovalbumin(OVA),and randomly divided into the model group,low-dose(54 mg/kg)asiatic acid group,high-dose(108 mg/kg)asiatic acid group,high-dose(108 mg/kg)asiatic acid+Colivelin(IL-6/STAT3/NF-κB activator,2 mg/kg)group,with 10 mice in each group,another 10 mice were selected and given equal doses of physiological saline during the sensitization and stimulation stages as the control group.Pathological morphology of pulmonary tissue,number of inflammatory cells in bronchoalveolar lavage fluid(BALF),levels of Treg/Th17 in peripheral blood,levels of Treg,Th17 cell secretion factors in BALF and serum,and expression of IL-6/STAT3/NF-κB pathway related proteins in pulmonary tissue were detected after treating with asiatic acid and Colivelin.Results:Compared with the control group,the pulmonary tissue of mice in the model group showed great pathological damage,the proportion of Treg cells and Treg/Th17 in peripheral blood,levels of IL-10 and IL-35 in BALF and serum levels decreased(P<0.05),the pulmonary inflammation score,white blood cell count and eosinophil count in BALF,proportion of Th17 cells in peripheral blood,levels of IL-17 and IL-6 in BALF and serum,IL-6 protein expression and p-STAT3/STAT3,p-NF-κB p65/NF-κB p65 in pulmonary tissue increased(P<0.05).Low-dose and high-dose asiatic acid reversed the above pathological changes in asthmatic mice models,and the effect of asiatic acid was stronger at higher doses.Colivelin could attenuate the pathological changes mentioned above in asthma model mice induced by asiatic acid.Conclusion:Asiatic acid can inhibit the production of inflammatory cells and inflammatory factors by decreasing the expression of IL-6 and the phosporylation of STAT3 and NF-κB in

关 键 词：积雪草酸 白细胞介素-6/信号转导子和转录激活子3/核转录因子-κB 哮喘 Treg/Th17 免疫平衡 

分 类 号：R285.5[医药卫生—中药学]