基于上胚轴外植体的甘蓝高效再生体系  

Efficient Regeneration System for Brassica olerecea Based on Epicotyl Explants

作　　者：韩昔潮 张肖健 蔡旭 梁建丽[2] 王晓武[2] 朱克明[1] 武剑[2] HAN Xichao;ZHANG Xiaojian;CAI Xu;LIANG Jianli;WANG Xiaowu;ZHU Keming;WU Jian(College of Life Sciences,Jiangsu University,Zhenjiang 212013,Jiangsu,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,State Key Laboratory of Vegetable Biobreeding,Beijing 100081,China)

机构地区：[1]江苏大学生命科学学院,江苏镇江212013 [2]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点试验室,北京100081

出　　处：《中国蔬菜》2024年第9期44-50,共7页China Vegetables

基　　金：国家自然科学基金项目(32072593);中国农业科学院科技创新工程项目(CAAS-ASTIPIVFCAAS)。

摘　　要：下胚轴是甘蓝遗传转化中常用的外植体,但基因型依赖和再生效率低是目前的主要问题。为探索以甘蓝上胚轴为外植体构建遗传转化体系,以9个甘蓝商品种为试材,比较了上胚轴与下胚轴两种外植体在农杆菌侵染后诱导分化的再生效率。结果表明:9个甘蓝品种中有5个上胚轴生长良好。在农杆菌侵染后,以上胚轴为外植体5个品种均诱导产生了不定芽,再生频率为32.4%~78.4%;而以下胚轴为外植体只有3个品种分化出不定芽,且最高再生频率仅为2.7%。表明上胚轴是克服甘蓝转基因再生体系中基因型依赖的理想外植体,为获得甘蓝再生植株提供了一种有效途径。The hypocotyl is a commonly used explant for genetic transformation of cabbage(Brassica oleracea var.capitata),however,the problems of genotype-dependent and low regeneration efficiency are the main obstacles at present.This study explores the construction of a genetic transformation system using the epicotyl of cabbage as an explant,with 9 cabbage varieties as test materials.The regeneration efficiency after Agrobacterium infection was compared between the hypocotyl and epicotyl as two explants.The results show that 5 cabbage varieties had elongated epicotyls.After Agrobacterium inoculation,all 5 varieties with epicotyls as explant induced adventitious shoots with regeneration frequencies ranging from 32.4% to 78.4%.In contrast,only 3 varieties induced of adventitious buds with hypocotyl as explant,with the highest regeneration frequency of only 2.7%.This shows that the epicotyl is an ideal explant for overcoming genotypic dependence in B.olerecea transformation,and provides an efficient approach to obtain regenerated B.oleraceaplants.

关 键 词：甘蓝 上胚轴 下胚轴 组织培养 遗传转化 

分 类 号：S635[农业科学—蔬菜学]