当归芍药散介导氧化-炎症通路在老年痴呆大鼠模型神经损伤保护中的机制探究  

Protection mechanism of Danggui Shaoyao Powder on nerve injury in rats with senile dementia by mediating oxidation-inflammation pathways

作　　者：王言 刘传凤 蒲琳[1] 文清华 WANG Yan;LIU Chuanfeng;PU Lin(Chengdu Hospital of Integrated Traditional Chinese and Western Medicine,Chengdu Sichuan 610041,China;Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu Sichuan 610072,China)

机构地区：[1]成都市中西医结合医院,四川成都610041 [2]成都中医药大学附属医院,四川成都610072 [3]重庆市石柱县人民医院,重庆石柱409199

出　　处：《四川中医》2024年第9期61-66,共6页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:探讨当归芍药散介导氧化-炎症通路在老年痴呆大鼠模型神经损伤保护中的机制。方法:72只大鼠随机分成对照组、模型组、当归芍药散低剂量组(12g/kg)、当归芍药散中剂量组(24g/kg)、当归芍药散高剂量组(36g/kg)和尼莫地平组(20mg/kg),每组12只。除对照组外,其余组大鼠均复制老年痴呆模型。建模成功后,当归芍药散不同剂量组和尼莫地平组分别灌胃不同剂量当归芍药散、尼莫地平。其余组灌胃等剂量的生理盐水,连续灌胃4周。Morris水迷宫实验检测空间学习记忆能力。高效液相色谱-电化学检测法海马组织间液去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)和5-羟吲哚乙酸(5-hydroxyindole acetic acid, 5-HIAA)水平。Longa法评估各组大鼠神经功能缺陷。酶联免疫吸附检测脑组织白介素1β(IL-1β)、白介素10(IL-10)、肿瘤坏死因子α(TNF-α)、超氧岐化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物(GSH-Px)。结果:模型组逃避潜伏期均短于对照组(P<0.05)。与模型组相比,当归芍药散不同剂量组和尼莫地平组逃避潜伏期均下降(P<0.05)。与对照组相比,模型组大鼠的神经功能缺损评分以及脑组织中TNF-α、IL-1β、IL-6水平升高(P<0.05)。当归芍药不同剂量组和尼莫地平组神经功能缺损评分以及脑组织中TNF-α、IL-1β、IL-6水平低于模型组(P<0.05)。模型组大鼠海马组织间液NE、DA、5-HT、5-HIAA水平以及脑组织SOD和GSH-Px含量均低于对照组,脑组织MDA含量高于对照组(P<0.05)。与模型组相比,当归芍药散不同剂量组和尼莫地平组大鼠海马组织间液NE、DA、5-HT、5-HIAA水平以及脑组织SOD和GSH-Px含量均升高,脑组织MDA含量降低(P<0.05)。与对照组相比,Nrf2表达下降,TLR4和NF-κB表达升高(P<0.05)。与模型组相比,当归芍药散不同剂量组和尼莫地平组Nrf2表达升高,TLR4和NF-κB表达下降(P<0.05)。结论:当归芍药散通过Nrf2/TLR4/NF-κB信�Objective To explore the protection mechanism of Danggui Shaoyao Powder on nerve injury in rats with senile dementia by mediating oxidation-inflammation pathways.Methods A total of 72 rats were randomly divided into control group,model group,Danggui Shaoyao Powder low-dose group(12g/kg),medium-dose group(24g/kg),high-dose group(36g/kg)and nimodipine group(20 mg/kg),12 cases in each group.Except control group,models of senile dementia rats were replicated in the other groups.After successful modeling,all Danggui Shaoyao Powder groups and nimodipine group were given intragastric administration of different doses of Danggui Shaoyao Powder and nimodipine,while the other groups were given intragastric administration of the same dose of normal saline for 4 weeks.The spatial learning and memory ability were detected by Morris water maze test.The levels of norepinephrine(NE),dopamine(DA),5-hydroxytryptamine(5-HT)and 5-hydroxyindole acetic acid(5-HIAA)in hippocampal interstitium fluid were detected by high performance liquid chromatography-electrochemical detection.The neurological deficits were evaluated by Longa method.The levels of interleukin-1β(IL-1β),interleukin-10(IL-10),tumor necrosis factorα(TNF-α),superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)in brain tissues were detected by enzyme-linked immunosorbent assay(ELISA).Results The escape latency in model group was shorter than that in control group(P<0.05).Compared with model group,escape latency was shortened in all Danggui Shaoyao Powder groups and nimodipine group(P<0.05).Compared with control group,score of neurological deficits and levels of TNF-α,IL-1βand IL-6 in brain tissues were increased in model group(P<0.05).The score of neurological deficits and levels of TNF-α,IL-1βand IL-6 in all Danggui Shaoyao Powder groups and nimodipine group were lower than those in model group(P<0.05).The levels of NE,DA,5-HT and 5-HIAA in hippocampal interstitium fluid,and levels of SOD and GSH-Px in brain tissues in model group were l

关 键 词：当归芍药散 氧化-炎症通路 老年痴呆 神经损伤 

分 类 号：R285.5[医药卫生—中药学]