中药药理学体外实验方法研究进展  

In Vitro Experimental Methods in Pharmacological Research of Chinese Materia Medica:A Review

作　　者：张秋菊[1,2] 李能莲 王宇[1,2] 金华[1,2] ZHANG Qiuju;LI Nenglian;WANG Yu;JIN Hua(Gansu University of Chinese Medicine,Lanzhou 730000,China;Key Laboratory of Dunhuang Medicine,Ministry of Education,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学,兰州730000 [2]敦煌医学与转化教育部重点实验室,兰州730000

出　　处：《中国实验方剂学杂志》2024年第20期283-289,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81860832,82160886);甘肃省自然科学基金项目(21JR7RA566);甘肃省联合科研基金项目(23JRRA1524);甘肃省省级重点人才项目(甘组通字[2023]20号);甘肃省中医药研究中心专项(zyzx-2020-zx7);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金项目(zzy-2022-05)。

摘　　要：体外实验是中药药理学研究不可或缺的方法,但由于中药来源广泛、成分多样、理化性质不稳定等因素阻碍了其体外实验的开展。因此,寻找更为科学、合理的体外药理学研究方法已成为亟待解决的问题。目前中药药理学体外实验常用的方法有直接给药法和间接给药法,间接给药法又包括含药血清/浆法、含药肠吸收液法、含药肝孵育液法等。随着计算机技术和系统生物学的发展,将高效液相色谱-质谱法(HPLC-MS)、组学、网络药理学、计算机/人工智能辅助药物设计等技术与中药体外实验方法相结合,不仅大大降低了科研实验成本,还明显提高了中药药理作用探索、有效成分筛选及药物研发的效率,已逐渐被认可并广泛应用。该文就目前常用的中药药理学体外实验方法及其进展做一梳理,以期为选择合理的研究方法提供帮助。In vitro experiments are an indispensable method in the pharmacological research of Chinese materia medica.However,the development of in vitro experiments is hindered by the various sources,complex composition,and unstable physical and chemical properties of Chinese materia medica.Therefore,it has become an urgent problem to develop reasonable methods for in vitro pharmacological research.At present,the commonly used in vitro experimental methods in the pharmacological research of Chinese materia medica include direct administration and indirect administration.Indirect administration methods include drug-containing serum/plasma,drug-containing intestinal absorption solution,and drug-containing liver incubation solution.With the development of computer technology and systems biology,the combination of high performance liquid chromatography-mass spectrometry(HPLC-MS),omics,network pharmacology,computer/artificial intelligence-assisted drug design and other technologies with in vitro experiments of Chinese materia medica has not only greatly reduced the research cost but also significantly improved the efficiency of pharmacological exploration,effective ingredient screening,and drug discovery.Therefore,the combined methods have been gradually recognized and widely used.This paper reviewed the in vitro experimental methods in pharmacological research of Chinese materia medica and their progress,aiming to provide help for selecting reasonable research methods.

关 键 词：中药药理学 体外实验 含药血清/浆 含药肠吸收液 含药肝孵育液法 

分 类 号：R2-0[医药卫生—中医学] R33[文化科学—情报学] G353.11R289